癌痛消颗粒对移植性肝癌模型大鼠缺氧微环境中细胞因子HIF-α mRNA及蛋白表达的影响  

Effects of Aitongxiao Granules on expression of mRNA and protein of cytokines HIF-α under hypoxic environment in rats with transplanted liver cancer

作　　者：高连印[1,2] 韦艾凌[3] 潘哲[3] 张永琴[3] 

机构地区：[1]湖南中医药大学,长沙410208 [2]首都医科大学中医药学院,北京100069 [3]广西中医药大学,南宁530001

出　　处：《北京中医药》2014年第3期223-225,共3页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81160438)

摘　　要：目的观察癌痛消颗粒对移植性肝癌模型大鼠缺氧微环境低氧诱导因子1α(HIF-α)的mRNA及蛋白表达的影响,探讨癌痛消颗粒抗肝癌作用的机制。方法将35只大鼠随机抽取5只作为正常组,其余30只为造模组,造模过程中死亡5只大鼠,造模成功后的移植性肝癌模型大鼠随机分为5-氟尿嘧啶组(5-Fu组),癌痛消颗粒高、中、低剂量组(药物浓度150、75、27.5 mg/mL),模型组。癌痛消颗粒高、中、低剂量组及模型组每日按照10 mL/kg体重灌胃给药治疗,连续用药14天后停药;5-Fu组按75 mg/kg体重腹腔注射治疗,每隔2天腹腔注射1次,连续注射5次,停药24 h后处死各组大鼠,取出瘤块,采用实时定量PCR(PT-PCR)、Western印迹技术观察HIF-αmRNA及蛋白的表达水平。结果癌痛消颗粒高、中、低剂量组及5-Fu组、模型组HIF-αmRNA及蛋白的表达水平与正常组比较差异有统计学意义(P<0.05);癌痛消颗粒高、中、低剂量组及5-Fu组HIF-αmRNA及蛋白表达水平与模型组比较差异有统计学意义(P<0.05),癌痛消颗粒中、低剂量组与5-Fu组比较差异有统计学意义(P<0.05),癌痛消颗粒高剂量组与5-Fu组比较差异无统计学意义(P>0.05)。结论癌痛消颗粒对移植性肝癌模型大鼠的肝癌有一定的治疗作用,其抗癌机制可能与降低HIF-αmRNA及蛋白的表达有关,且癌痛消颗粒高剂量组的疗效最好。Objective To observe the effects of Aitongxiao Granules on expression of mRNA and protein of HIF-ct under hypoxic environment in rats with transplanted liver cancer. Methods Among thirty-five rats, 5 rats were randomly assigned into a normal group and the rest of 30 rats were into model group. During the process of model establishment 5 rats were dead. After model establishment, rats with transplanted liver cancer were randomly divided into a 5-fluorouracil （5-Fu） group, a high dose group （150 mg/mL）, a medium dose group （75 mg/mL）, a low dose group （27. 5 mg/mL） and a model group. According to 10 mL/kg, intragastric administration of the granules was applied in the high dose group, medium dose group, low dose group and model group, once a day for continuous 14 days. According to 75 mL/kg, intraperitoneal injection was applied in the 5-Fu group, once every two days for 5 times. 24 h after the end of treatment, rats in each group were sacrificed to collect their tumors. The real-time quantitative PCR and Western Blot method were applied to observe the expression of mRNA and protein of HIF-a. Results The expression of mRNA and protein of HIF-a in the 5-Fu group, high dose group, medium dose group, low dose group and model group was statistically different from that in the normal group （ P 〈0. 05）. The expression of mRNA and protein of HIF-ot in the 5-Fu group, high dose group, medium dose group and low dose group was statistically different from that in the model group （P 〈 0. 05 ）. The Results in the medium dose group and low dose group were statistically different from that in the 5-Fu group （P 〈0. 05）, but those in the high dose group were not statistically different from that in the 5-Fu group （P 〉0. 05）. Condusion The Aitongxiao Granules has positive therapeutic effects on rats with transplanted liver cancer, which could be related with lowering the expression of mRNA and protein of HIF-a; the high dose of Aitongxiao Granules has the best efficacy.

关 键 词：肝癌 癌痛消 低氧诱导因子1a 

分 类 号：R273[医药卫生—中西医结合]