调节性T细胞在人乳头瘤病毒16整合状态的宫颈癌组织中的表达及意义  

The expression of Treg in HPV16 integrated cervical cancer

作　　者：丁爱萍[1,2] 张志新[3] 张瑶[1] 魏恒[1] 张淑兰[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004 [2]常州市妇幼保健院妇产科,江苏常州213000 [3]中国人民解放军第102医院骨科,江苏常州213000

出　　处：《中国实用妇科与产科杂志》2014年第5期374-378,共5页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：国家自然科学基金资助项目(81372776);辽宁省医学高峰建设工程项目(2010696);辽宁省科学技术计划项目(2011225009);盛京自由研究者计划(201302);高等学校博士学科点专项科研基金(20122104110014)

摘　　要：目的探讨调节性T细胞(Treg)活化与人乳头瘤病毒(HPV)16存在状态的关系及其与HPV16整合状态的宫颈鳞癌患者临床病理资料之间的相关性。方法 2012年1-10月在中国医科大学附属盛京医院妇产科,采用多重实时PCR技术对HPV16阳性的17例正常宫颈组织、65例宫颈上皮内瘤变(CIN)、60例宫颈鳞癌(CC)的宫颈脱落细胞标本检测E2和E6基因,通过E2/E6比值法评估HPV16 DNA的体内存在状态。采用免疫组化方法检测相应宫颈组织中叉头状/翅膀状螺旋转录因子(Foxp3)的表达。结果 Foxp3阳性积分光密度值在HPV16游离状态、整合状态宫颈组织中,均与宫颈病变严重程度呈正比,在CC与CINⅢ组比较差异有统计学意义(P<0.05)。在同一病变中,HPV16整合型组Foxp3阳性表达均高于游离型组,在CC组比较差异有统计学意义(t=-2.685,P<0.05)。Foxp3阳性在HPV16整合状态宫颈鳞癌中的表达与FIGO分期、组织学分级及淋巴结转移相关(P<0.05),而与年龄无关(P>0.05)。结论 Treg与HPV存在状态密切相关,Treg在HPV持续感染所致宫颈癌的发生及发展中可能起着重要作用。Objective To analyze the relationship between Treg activation and the existence state of HPV, as well as the relationship between Treg activation and clinicopathological features of integrated cervical cancer. Methods Between January 2012 and October 2012, in Shengjing Hospital of China Medical University, cervical liquid-based cytological （ LBC ） samples from patients with normal cervical uteri （ n = 17 ）, CIN tissues （ n = 65 ）, cervical squamous cancer（ n = 60） were detected E2 and E6 genes of HPV type 16 using multiple polymerase chain reaction （PCR）. E2/E6 ratio was used to evaluate the physical status of HPV-16 DNA in host cell genome. The SP immunohistochemical method was used to detect the expressions of Foxp3 in all cases. Results Foxp3＋ integrated optical density value in the HPV16 free, integrated cervical tissue were increased with the severity of cervical lesions increased, CC and CINIII group was more statistically significant （ P 〈 0.05 ）. In the same lesions, integrated group of Foxp3＋ expression higher than free type group, in the CC group was more statistically significant than CIN （ t = - 2. 685, P 〈 0. 05 ）. The expression of Foxp3＋ in integrated cervical squamous carcinoma was correlated with FIGO stages, histological grade, lymph node metastasis （ P 〈 0. 05 ） and is independent of age （ P 〉 0.05 ）. Conclusion Treg is closely related to HPV persistence,Treg may play an important role in the occurrence and development of cervical cancer caused by HPV persistent infection.

关 键 词：宫颈鳞癌 调节性T细胞 HPV16 病毒整合 

分 类 号：R711.74[医药卫生—妇产科学]