线性约束的混料设计优化双水相提取红梅消总皂苷工艺  

Optimization of Extraction Process for Total Saponins from Rubus parvifolius in Aqueous Two-Phase System by Mixture Design

作　　者：周文杰[1,2] 杨荣平[2] 禹奇男 柯秀梅[1,2] 刘楠[1,2] 励娜[2] 

机构地区：[1]成都中医药大学成都611137 [2]重庆市中药研究院,重庆400065

出　　处：《中国实验方剂学杂志》2014年第9期32-35,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：重庆市科技计划项目(cstc2012pt-kyys10004)

摘　　要：目的:优化双水相提取红梅消总皂苷的工艺条件。方法:以齐墩果酸为指标成分,采用超声耦合乙醇-硫酸铵双水相体系提取红梅消总皂苷,采用线性约束的混料设计,优化双水相体系中硫酸铵、无水乙醇和水的质量分数,运用多元线性回归及二项式拟合建立指标与因素间的数学模型,预测红梅消总皂苷最佳提取条件。结果:最优双水相提取条件为硫酸铵11%,无水乙醇37%,水52%;总皂苷提取率3.10%,与预测值3.14%的相对误差-1.27%。结论:线性约束的混料设计适用于双水相提取红梅消总皂苷工艺的优化,建立的数学模型预测性良好。Objective：To optimize extraction process of total saponins from Rubus parvifolius in aqueous two-phase system.Method：Taking oleanolic acid as index component,ultrasound-assisted coupling ethanolammonium sulfate aqueous two-phase system was adopted to extract total saponins.Mixture design in linear constraints was employed with three independent variables including amounts of ammonium sulfate,ethanol and water,while the content of total saponins as dependent variable,mathematical model between index and factors was established by multi-linear regression and binomial fitting,then optimum process conditions were predicted.Result：Optimum process conditions were as follows：ammonium sulfate 11％,ethanol 37％,water 52％ ;Extraction rate of total saponins was 3.10％,which had relative error of-1.27％ with the predicted value of 3.14％.Conclusion：Mixture design facilitated to optimize extraction technology of total saponins from R.parvifolius in aqueous two-phase system,this established model had good predictability.

关 键 词：红梅消 总皂苷 双水相体系 提取工艺 混料设计 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]