壮药大头陈中桦木酸的分离鉴定及含量测定  

Separation and Determination of Betulinic Acid in Adenosma indianum(Lour.) Merr. by HPLC

作　　者：陆国寿[1] 黄秋洁[2] 叶勇[3] 卢文杰[1,4] 牙启康[1] 黄伟城[3] 

机构地区：[1]广西中医药研究院,南宁530022 [2]广西中医药大学,南宁530001 [3]广西医科大学,南宁530021 [4]广西中药质量标准研究重点实验室,南宁530022

出　　处：《中国实验方剂学杂志》2014年第9期90-92,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西卫生厅中医药科技专项(GZKZ1135)

摘　　要：目的：对大头陈中的化学成分进行研究;采用HPLC法对大头陈中桦木酸的含量进行测定.方法：采用溶剂提取、萃取、硅胶色谱柱分离纯化,利用色谱技术进行化学结构鉴定;HPLC法进行含量测定的色谱条件为：采用SHISEIDO-SPOLAR C18（4.6mm×250mm,5μm）,流动相甲醇-0.2％磷酸水溶液（82∶ 18）,流速1.0 mL· min-,检测波长205 nm,柱温35℃.结果：从大头陈药材中分离得到一化合物,经鉴定为桦木酸;桦木酸在0.048 ～0.48 μg与其峰面积呈良好的线性关系;平均回收率99.84％,RSD 1.35％.结论：该化合物首次从该植物中分离得到.所建立的HPLC简便、准确、重复性好.结果准确可靠,可用于大头陈中桦木酸的测定.Objective：To study the chemical constituents of Adenosma indianum （Lour.） Merr.and the contents of betulinic acid in Adenosma indianum （Lour.） Merr.by HPLC.Method：The chemical constituents were isolated by column chromatography over silica ge1.The Structures of the compound was elucidated by spectroscopic and chromatographic analysis.SHISEIDO-SPOLAR C18 column （4.6 mm × 250 mm,5 μm） was used with methanol-0.2％ phosphoric acid （82∶ 18） as mobile phase.The flow rate was 1.0 mL ·min^-1.The UV detection wavelength was 205 nm and the column temperature was 35 ℃.Result：The compound was identified as betulinic acid.The linear response range of betulinic acid were 0.048-0.48 μg,respectively.The average recovery of this compound was 99.84％,RSD 1.35％.Conclusion：The betulinic acid was isolated from Adenosma indianum （Lour.） Merr.for the first time.This established method in this study is simple,reliable,reproducible and accurate for the analysis of betulinic acid in Adenosma indianum （Lour.） Merr.

关 键 词：大头陈 桦木酸 球花毛麝香 

分 类 号：R284.1[医药卫生—中药学]