鬼箭羽总黄酮含量测定方法的建立  

Optimization of Determination for Total Flavonoids in Euonymus alatus

作　　者：党晓芳[1] 曹飒丽[1] 祁娟娟[1] 张慧[1] 张晋[1] 付京[1] 陈杨[1] 倪健[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2014年第9期96-98,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京中医药大学复方中药制药创新团队项目(2011-CX70-13)

摘　　要：目的：建立鬼箭羽总黄酮的含量测定方法并筛选其提取方法.方法：以芦丁为对照品,通过单因素试验优选显色方法和显色条件,建立总黄酮含量测定的方法学考察并优选其提取方法.结果：采用盐酸-镁粉反应比色法,芦丁在0.020～0.104 mg与吸光度呈良好线性关系（r=0.997）,平均加样回收率99.424％（RSD 2.220％）.结论：盐酸-镁粉显色法具有精密度高、重复性好等优势,适用于鬼箭羽总黄酮的定量分析.鬼箭羽总黄酮的提取宜采用回流法.Objective：To establish a method for determination of the content of total flavonoids in Euonymus alatus.Method：Selecting rutin as a reference substance,single factor tests were adopted to optimize colorimetry methods and conditions,determination of the content of total flavonoids was established and optimized its extraction method.Result：Hydrochloric acid-Mg reaction assay was adopted,rutin had a liner relationship with absorbance in the range of 0.021-0.104 mg （r =0.997）,average recovery was 99.424％ with RSD of 2.220％.Conclusion：HCl-Mg colorimetry method appeared to be an accurate,reliable and repeatable system for determination of total flavonoids in E.alatus.Total flavonoids in E.alatus should employ reflux extraction method.

关 键 词：鬼箭羽 总黄酮 显色条件 单因素试验 方法学考察 提取方式 

分 类 号：R283.6[医药卫生—中药学]