化痰通络方对急性脑梗死大鼠rt-PA溶栓后不同脑区星形胶质细胞特异性蛋白表达的影响  

Effects of Huatan Tongluo Decoction on Astrocytes Related Specific Protein Expression of Different Brain Tissues in Rats Which Use rt-PA Thrombolysis after Acute Cerebral Infarction

作　　者：周震[1] 王凯[2] 刘爽[1] 宋宛珊[2] 马妍[2] 田涛涛[2] 张玉莲[1] 

机构地区：[1]天津中医药大学第二附属医院,天津300150 [2]天津中医药大学,天津300193

出　　处：《中国实验方剂学杂志》2014年第9期184-189,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072796)

摘　　要：目的:观察化痰通络方对急性脑梗死大鼠重组组织纤溶酶原激活剂(rt-PA)溶栓后不同脑组织中星形胶质细胞相关特异性蛋白水通道蛋白4(AQP-4)及血清中S100β蛋白表达的影响。方法:将240只健康SD大鼠随机分为6组:假手术组、模型组、rt-PA组、化痰通络方联合rt-PA组(中药低、中、高剂量组,剂量分别为3.6,7.2,14.4 g·kg-1),采用自身栓子法制备大鼠大脑中动脉栓塞模型(MCAO),rt-PA组与化痰通络方联合rt-PA组于血栓注入后3 h一次性予以5.67 mg·kg-1rt-PA溶栓治疗,后者联合给予低、中、高剂量化痰通络方中药灌胃治疗,6 h组于造模成功后给予中药1次;24 h组于造摸成功后及处死前2 h各给中药1次,共2次;72 h组于造模成功后开始给药,每日给中药2次,持续3 d,共6次;7 d组于造模成功后开始给药,每日给中药2次,持续7 d,共14次。分别于4个时相应用Western blot法观察不同时相大鼠皮质及海马AQP-4蛋白的表达,ELISA法检测血清中S100β蛋白的含量。结果:各实验组不同脑组织AQP-4相对丰度值及血清S100β含量自6 h起逐渐升高,24 h最高,随后逐渐降低,7 d时接近6 h水平;同一时相与假手术组比较,各实验组AQP-4及S100β蛋白均显著升高,说明造模成功。与模型组相比,中药中、高剂量组AQP-4蛋白于24 h或72 h或7 d时分别出现显著下降(P<0.05),研究结果并无明显集中现象,但整体趋势表明中药中、高剂量组能明显减少AQP-4的表达;rt-PA组、中药低、中、高剂量组S100β虽未出现明显差异,但均有不同程度降低,且以中药组下降趋势明显。结论:①溶栓治疗后,血脑屏障结构及功能损害于24 h时最为严重;②化痰通络方联合rt-PA溶栓可降低不同脑组织中AQP-4表达及血清中S100β含量进而防治急性脑梗死溶栓后出血转化的发生与发展。Objective：To observe the effect of Huatan Tongluo decoction on aquaporin-4 （AQP-4） protein expression of different brain tissues and S100β of blood serum in rats which use rt-PA thrombolysis after the acute cerebral infarction.Method：SD rats 240 were randomly divided into six groups,sham operation group,model group,rt-PA group,rt-PA Huatan Tongluo of Chinese medicine low dose group （3.6 g ·kg^-1）,rt-PA Huatan Tongluo of Chinese medicine middle dose group （7.2 g · kg^-1）,rt-PA Huatan Tongluo of Chinese medicine high dose group （14.4 g ·kg^-1）.Using the embolus method prepares the MCAO rat model.rt-PA group and rt-PA Huatan Tongluo group use rt-PA thrombolytic therapy after thrombosis.In 6 hours group the Chinese medicine were given one time,after making mode successfully.In 24 hours group,given two times,after making mode successfully and 2 hours were killed.In 72 hours,given six times,from making mode successfully,two times one day,lasting three days.In 7 day group; given fourteen times,from making mode successfully,two times one day,lasting seven days.Then respectively observe the AQP-4 protein expression in rat cortex and hippocampus by Western blot method,and S1O0β of blood serum by ELISA method at 6,24,72 h,7 days.Result：Every experimental group in different phases,the AQP-4 protein expression in different brain tissue and S100β of blood serum were highest at 6,24 h maximum,72 h,7 d increased gradually to the level of 6 h.Compared with the model group at the same time the AQP-4 protein expression of middle dose group and high dose group of Chinese traditional medicine were significantly decreased at 24 h or 72 h or 7 d （P ＜ 0.05）.There was no obvious concentration,but the overall trend showed that Chinese medicine middle dose group and high dose group can obviously decrease the expression of AQP-4.The S100β protein expression didn＇t show any significant differences in any group,but all have reduced in different degree,and the downward trend is more obvious in Chinese tradi

关 键 词：化痰通络方 急性脑梗死 溶栓 出血转化 水通道蛋白4 S100Β蛋白 

分 类 号：R285.5[医药卫生—中药学]