病毒性心肌炎(病)患者中IFNγ和IL10的表达及药物干预的研究  

The Study on the Expression of IFN-γ and IL-10 in Patients with Viral Myocarditis and their Interference with Chinese Drugs

作　　者：陈曙霞[1] 徐欣辉[1] 张冬青[2] 梅尚文[1] 谢龙山[1] 陈美芳[1] 钱富荣[1] 

机构地区：[1]上海第二医科大学附属仁济医院,上海200001 [2]上海第二医科大学上海市免疫学研究所,上海200025

出　　处：《上海免疫学杂志》2001年第1期14-17,共4页Shanghai Journal of Immunology

基　　金：国家"九五"医技攻关基金!资助项目 (部分内容 ) 96 90 6 0 2 137

摘　　要：本研究采用ELISA法检测心肌炎 (病 )患者外周血Th1/Th2相关细胞因子IFN γ及IL 10浓度 ,以RT PCR检测柯萨奇B病毒 (CVB )表达情况 ,并观察“抗柯”注射液给药后的效果。结果表明 ,(1)急性期者 ,CVB RNA阳性者居多 ,Th1均增高 ,Th2类细胞因子IL 10正常或偏低 ;(2 )恢复期者 ,CVB RNA阴性者居多 ,IL 10均增高 ,IFN γ正常或偏低 ;(3)慢性期者 (包括扩张型心肌病 )Th1/Th2均降低 ,内含CVB RNA持续阳性者。表明心肌炎的炎症主要是Th1细胞所介导 ,而Th2类细胞因子对其有抑制作用 ,抗柯注射液提高Th2活性 ,抑制Th1活性。To study the cytokine profiles of lymphocytes from peripheral blood of patients with myocarditis and cardiomyopathy,the concentrations of Th1/Th2 associated cytokines IFN γ and IL 10 were determined by ELISA,and the expressions of coxsackie B virus(CVB)genes were tested by RT PCR assay in comparison with those of the normal contact individuals Meanwhile,the effect of Kang Ke injections was also observed It was found that most of patients had a positive CBV RNA reactions in acute stage of illness with increased production of Th1 related cytokine IFN γ and normal or reduced production of Th2 related cytokine IL 10 Neverthless,in convalescent stage,most patients showed a negative CVB RNA reactions with increased production of IL 10 and normal or reduced response of IFN γ.In chronic stage (including those patients with dilated cardiomyopathy),both IFN γ and IL 10 were depressed These results suggest that the inflammatory reactions in myocarditis are mainly manifested through the Th1 cell mediated responses,and the Th2 cell type cytokines have an inhibitory effect on these responses The Kang Ke injections can enhance the Th2 cell activities,but can inhibit the Th1 cell activities

关 键 词：患病人群 病毒性心肌炎 扩张型心肌病 表达 IFN-Γ IL-10 

分 类 号：R542.21[医药卫生—心血管疾病]