葡萄糖分子印迹电化学传感器的研制及其应用  

The Fabrication of Glucose Molecular-Imprinted Electrochemical Sensor and Its Application

作　　者：付丛[1] 韦宏伟[1] 杜娟 李建平[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541004 [2]桂林市环境监测中心站,广西桂林541002

出　　处：《分析科学学报》2014年第2期181-185,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21165007);广西自然科学基金(No.2012GXNSFAA053032)

摘　　要：本文结合分子印迹技术与电化学检测手段,制备了高选择性、高灵敏度和价格低廉的分子印迹电化学传感器,并利用该传感器对食品中的葡萄糖进行定量分析。由于该分子印迹膜为非导电膜,本实验以铁氰化钾-亚铁氰化钾离子作为底液与电极之间的探针并通过铁氰化钾-亚铁氰化钾氧化还原电流信号的变化来对葡萄糖浓度进行间接测定。实验结果表明,在0.01~2μmol/L的范围内,葡萄糖浓度的变化与铁氰化钾-亚铁氰化钾氧化还原电流信号变化呈线性关系,检出限为7.68×10-9 mol/L。该传感器的制备和测量方法简单,可用于实际样品的检测。A highly sensitive,highly selective and low cost sensor for glucose determination was prepared based on the molecular-imprinted technique and electrochemical detection method. The sensor was used in the determination of glucose in beer and apple samples. In the assay, the sensor was characterized by cyclic voltammetry method in K3 [-Fe（CN）6 ] solution. The glucose concentration can be determined in the range of 0.01-2 μmol/L with a detection limit of 7.68× 10-9 mol/L by recording the redox currents of K3[Fe（CN）6/K4 [Fe（CN）6]. The sensor is simple and convenient in the process of fabrication and determination, and shows good reproducibility and recovery in real sample determination.

关 键 词：葡萄糖 分子印迹电化学传感器 门效应 

分 类 号：O657.15[理学—分析化学]