光谱及电化学方法研究头孢哌酮钠和盐酸环丙沙星与人血清白蛋白的拮抗作用  

Studies on the Interaction of Antibiotics with Human Serum Albumin by Spectroscopic and Electrochemical Methods

作　　者：傅丽[1] 王树军[1] 靳红利 张世乾 孟雅男 

机构地区：[1]廊坊师范学院化学与材料科学学院,河北廊坊065000

出　　处：《分析科学学报》2014年第2期239-243,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.20975029);廊坊师范学院科学研究项目重点专项基金(LSZY201103)

摘　　要：在模拟生理条件下,用荧光光谱、紫外吸收光谱并结合电化学方法,研究了头孢哌酮钠(CPZ)和盐酸环丙沙星(CPFX)单独存在与同时存在时与人血清白蛋白(HSA)的相互作用。结果表明:药物与HSA的作用力主要为静电力,HSA的荧光猝灭为静态猝灭;药物与HSA结合位点数约为1;CPZ与CPFX间存在拮抗作用;拮抗作用使药物与HSA间的结合距离r值降低。电化学方法研究表明药物与蛋白相互作用形成了非电活性的的超分子化合物,使得溶液中游离的药物浓度降低。We investigated the interaction of medicines and HSA in cases that the two medicines cefoperazone sodium（CPZ） and ciprofloxacin hydrochloride（CPFX） coexisted and CPZ or CPEX was presented alone. The experiments have been made under simulated physiological conditions, and the data were acquired by use of fluorescence spectroscopy,UV absorption spectroscopy, as well as electrochemical methods. The results suggested that interaction of medicines and HSA was a reaction of the static quenching of chemical compound,and the binding force of medicine-HSA was mainly governed by electrostatic force. The number of binding site between medicines and HSA was approximately 1. There were antagonistic effects between CPZ and CPEX which made r value （the binding distance between medicines and HSA） decreased. Electrochemical study showed that drugs interacted with proteins to form the non-electric activity supramolecular compounds,which lowered the free drug concentration in the solution.

关 键 词：头孢哌酮钠 盐酸环丙沙星 人血清白蛋白 拮抗作用 光谱及电化学法 

分 类 号：O657.3[理学—分析化学]