液相色谱法测定地表水中氯酚类化合物  

Determination of Chlorophenols in Water by Liquid Chromatography

作　　者：付艳芳[1] 周建科[1] 李向梅[1] 

机构地区：[1]河北大学理化分析中心、河北省分析科学技术重点实验室,河北保定071002

出　　处：《分析科学学报》2014年第2期283-286,共4页Journal of Analytical Science

摘　　要：采用单滴液相微萃取-高效液相色谱法测定水中的4-氯酚、2,6-二氯酚、2,4-二氯酚和2,4,6-三氯酚。色谱条件为：Diamonsil C18柱（250×4.6mm i.d.,5μm）,柱温：室温,流速1.0mL/min,以甲醇∶水∶甲酸（体积比70∶30∶0.2）为流动相,225nm紫外检测。考察了萃取溶剂种类、萃取时间、萃取温度、搅拌速度和pH值对萃取效率的影响。4-氯酚、2,6-二氯酚、2,4-二氯酚在1.5~100mg/L和2,4,6-三氯酚在2.5~100mg/L范围内有良好线性,相关系数不低于0.9996,回收率在74.9%~84.4%之间,相对标准偏差低于4.9%。A method based on single-drop microextraction-liquid chromatography was used for the determination of chlorophenols in water samples. A Diamonsil C18 （250× 4.6 mm i. d. , 5 μm） was used0. 2,V/V/V） at a flow rate of 1.0 mL/min. The wavelengths of UV detector are 225 nm. The factors relevant to recoveries, such as type of extractant, extractant time, temperature, stiring speed and water samples pH were investigated and optimized. Four chlorophenols exhibited good linearity with the correlation coefficients above 0. 9996, with the average recoveries from 74. 9% to 84.4% and relative standard deviations less than 4.9 %.

关 键 词：单滴液相微萃取 反相液相色谱 二氯酚异构体 4-氯酚 2 4 6-三氯酚 

分 类 号：O657.72[理学—分析化学]