罗格列酮对2型糖尿病大鼠胰岛β细胞增殖与再生的影响  

Effects of rosiglitazone on proliferation and regeneration of islet beta cells in type 2 diabetic rats

作　　者：陈晓铭[1] 吴美芬[1] 郑坤杰[1] 方烁[1] 胡卓清 刘阳[1] 武革[1] 

机构地区：[1]广东医学院附属医院内分泌科,广东省湛江市524001

出　　处：《实用医学杂志》2014年第8期1193-1195,共3页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:2011B031800231);广东省医学科研基金立项课题(编号:B2011243);湛江市非资助科技攻关计划项目(编号:2013B01099)

摘　　要：目的:观察罗格列酮(RSG)对2型糖尿病大鼠胰岛β细胞增殖与再生的影响。方法:SD大鼠随机分为正常对照组、糖尿病组(DM组)和RSG组,每组各12只。DM组和RSG组给予高脂饲料喂养,于第8周末以链脲佐菌素30 mg/kg腹腔注射,成模后RSG组给予RSG 4 mg/(kg·d)灌胃持续4周。处死大鼠,取出胰腺组织,以免疫组织化学染色观察胰腺胰岛细胞的胰岛素(INS)、胰岛增殖细胞核抗原(PCNA)的表达变化,以原位末端标记法(TUNEL)检测胰腺胰岛细胞凋亡。结果:RSG组INS阳性表达率为75.0%,明显高于DM组(P<0.001);PCNA在RSG组胰腺组织的阳性表达率为66.7%,高于DM组(P<0.05);RSG组胰岛β细胞凋亡率明显降低(P<0.001)。结论:RSG可减少胰岛β细胞凋亡,促进其增殖。Objective To study the effects of rosiglitazone on proliferation and regeneration of islet β ceils in type 2 diabetes rats. Methods Thirty-six Sprague-Dawley （SD） rats were randomly divided into normal control group, diabetes mellitus （DM） group and rosiglitazone （RSG） group, 12 in each group. The experimental groups were injected by STZ 30 mg/（kg . bw） intraperitoneally for modeling in the eighth weekend. After modeling, the diabetic rats in the RSG group were lavaged with rosiglitazone 4 mg/kg once daily for 4 successive weeks. All rats were sacrificed after 4 weeks of medication and the pancreatic tissue was taken out immediately. The expression of islet insulin and islet proliferating cell nuclear antigen （PCNA） was detected by immunohistochemical staining. Apoptosis of islet cells was detected by TdT-mediated dUTP nick end labeling （TUNEL）. Results The positive expression rate of INS and PNCA in RSG group were significantly higher than that in DM group （P 〈 0.05）. Compared with the DM group, the level of apoptosis rate in the RSG group was significantly lower （P 〈 0.001 ）. Conclusion Rosiglitazone could decrease the islet β cells apoptosis rate and promote those proliferation.

关 键 词：糖尿病 2型 大鼠 胰岛Β细胞 凋亡 增殖 罗格列酮 

分 类 号：R587.1[医药卫生—内分泌]