两种植物内生真菌的醇提取物对水稻根部基因表达谱的分析  

Analysis of the Ethanol Extraction from Two Kinds of Cultured Endophytic Fungi to Rice Root Gene Expression

作　　者：王红[1] 杨镇[1] 肖军[1] 王娜[1] 肇莹[1] 陈珣[1] 龚娜[1] 曹君[1] 杨涛[1] 

机构地区：[1]辽宁省农业科学院微生物工程中心,辽宁沈阳110161

出　　处：《作物杂志》2014年第2期48-52,I0002,共6页Crops

基　　金：辽宁省科技攻关项目(2011215005)

摘　　要：两种植物内生真菌Coprinus micaccus和Alteranaria sp.的醇提取物可促进水稻根系发达,增加水稻产量,为了明确4种提取物对水稻全基因表达谱的影响,采用Affymetrix水稻芯片对水稻根系基因表达谱进行了分析,得到表达量同时发生变化的基因244个,其中109个基因上调表达,135个下调表达。基因按功能分为3大类,其中生物过程类归类为18个功能类别,多数差异基因与能量代谢、物质运输、生物合成及植物防御反应、信号转导、转录调节、蛋白质修饰等过程有关。引起11条代谢途径发生变化,使其向着更有利于植物生长发育的方向进行。The ethanol extract of two kinds of endophytic fungi,which were Coprinus micaccus and Alteranaria sp. , could promote the development of rice root system, increase rice yield. In order to analyse rice gene expression spec- trum,using the Gene Chip,there are 244 genes expression changed at the same time,which 109 gene up-regulated expession rasied, 135 down-regulation. The genes are divided into 3 categoris. The genes of biological process were classified into 18 functional groups and most of them related to energy metabolism, material transport, plant defence, signal transduction, transcriptional regulation, protein modification and so on. There are pathways changed, making it more conducive to the growth and development of plants biosynthesis, 11 metabolic

关 键 词：水稻 植物内真生菌 基因芯片 基因表达谱 代谢途径 

分 类 号：S511[农业科学—作物学]