甜荞叶片SOD、POD、CAT活性对矿质营养元素的响应  

The Response of Superoxide Dismutase, Peroxidase and Catalase Activities to Mineral Nutrition Elements in the Leaves of Buckwheat

作　　者：王淑敏[1] 盛晋华[1] 张雄杰[2] 杨丽君[1] 

机构地区：[1]内蒙古农业大学,内蒙古呼和浩特010019 [2]内蒙古天际绿洲特色生物资源研发中心,内蒙古呼和浩特010018

出　　处：《作物杂志》2014年第2期93-96,共4页Crops

基　　金：现代农业产业技术体系建设专项资金(CARS-08-B-5);内蒙古自治区自然科学基金项目(2011MS0305);内蒙古自治区应用技术研究与开发资金项目"荞麦新品种筛选及优质高产栽培技术集成研究"(2012);内蒙古教育厅"青年科技领军人才"(NJYT-13-A05);国家科技支撑计划课题(2014BAD07B05)

摘　　要：研究了矿质营养元素对甜荞品种温莎叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性的影响。试验采用三因素三水平正交试验设计,分别采用氮蓝四唑比色法(NBT)、愈创木酚法和紫外吸收法测定SOD、POD和CAT活性。结果表明:不同浓度的大量元素和微量元素对荞麦叶片SOD、POD、CAT活性均有不同程度的影响,在荞麦第二分枝现蕾初期,叶面分别喷施大量元素(0.15%硫酸钙+0.20%硫酸镁+0.25%磷酸二氢钾)和微量元素(0.15%硼砂+0.10%氯化锰+0.20%钼酸铵)对SOD、POD、CAT活性的提高尤为显著。Effect of mineral elements on the activity of superoxide dismutase （ SOD）, peroxidase （ POD）, catalase （CAT） was detected in the leaves of common buckwheat variety ＇ Wensha＇. In this study,orthogonal experimental design with three factors and three levels was taken. The activities of SOD, POD, and CAT were determined with methods of NBT, guaiacol and ultraviolet absorption. It was shown that diverse effect on SOD, POD, and CAT activi- ties of buckwheat leaves were detected with different concentration of major and trace elements. The SOD, POD, and CAT activities significantly increased with the treatments （0. 15% calcium sulfate, 0.20% magnesium sulfate, 0. 25 % monopotassium phosphate and 0. 15 % borax, 0. 10% manganese chloride, 0. 20% ammonium molybdate ） at the preliminary stage of second branch squaring.

关 键 词：甜荞 矿质元素 SOD POD CAT 

分 类 号：S517[农业科学—作物学]