南京典型设施菜地有机肥和土壤中四环素类抗生素的污染特征调查  

Pollution Characteristics of Tetracycline Antibiotics in Typical Protected Vegetable Organic Fertilizer of Nanjing City

作　　者：罗凯[1,2] 李文红[1] 章海波[2,3] 黄玉娟[2] 马婷婷[2] 吴龙华[2] 刘鸿雁[1,4] 骆永明[2,3] 

机构地区：[1]贵州大学农学院,贵阳550025 [2]中国科学院南京土壤研究所土壤环境与污染修复重点实验室,南京210008 [3]中国科学院烟台海岸带研究所海岸带环境过程重点实验室,山东烟台264003 [4]贵州大学资源与环境工程学院,贵阳550003

出　　处：《土壤》2014年第2期330-338,共9页Soils

基　　金：国家自然科学基金项目(41271326;40930739);环保公益性行业科研专项项目(201109018)资助

摘　　要：在南京市范围内采集谷里村、锁石村和东庐村3个地区的典型设施菜地有机肥及土壤样品，并利用超声波提取，固相萃取一高效液相色谱一串联质谱分析的方法测定了其中的四环素、土霉素、金霉素和强力霉素等4环素类抗生素。结果表明，采集的有机肥和土壤样品中四环素类抗生素均被检测出，其浓度以土霉素为最高，四环素和强力霉素其次，金霉素浓度较低。在调查的3个地区中，谷里村的有机肥中四环素类抗生素浓度最高，锁石村其次，东庐村则相对较低，各地区抗生素总量范围分别在126～8071、266～3326和339～373μg／kg，平均浓度分别为2152、1188和356μg／kg；不同种类的有机肥中抗生素的浓度差异很大，其中人畜粪便的抗生素总量为371～7820μg／kg；而对3个地区土壤中四环素类抗生素浓度的分析结果表明，谷里村仍为最高，总量范围为18．4～483μg／kg。由此可见，施用含有四环素类抗生素的有机肥已对土壤环境造成一定的威胁，有机肥农用的潜在问题应当引起关注。The occurrence and distribution of four selected antibiotics, include tetracycline （TC）, oxytetracycline （OTC）, chlortetracycline （CTC） and doxycycline （DOC）, in organic fertilizers were investigated at three typical protected vegetable fields in Guli, Suoshi and Donglu villages, suburb of Nanjing City. These four antibiotics were extracted by solvent, and detected by liquid chromatography-electro spray ionization tandem mass spectrometry （LC-ESI-MS/MS）. The results showed that TCs antibiotics were detectable in all the organic fertilizer samples. The highest concentration was OTC, TC and DOC were the second, and CTC had a lowest level. In those three different regions of Guli, Suoshi and Donglu, the concentration of which ranged from 126-8071,266-3326,339-373 μg/kg, and with the average of2152, 1188,356 μg/kg. The results also suggested that the concentration of antibiotics in different types of organic fertilizers were quite variable, especially the total concentrations of animal and human excreta, ranged from 371 to 7 820 μg/kg. Therefore, tetracyclines pollution from organic fertilizers might threaten to the soil environment, and need be concerned.

关 键 词：设施菜地 有机肥 四环素抗生素 污染特征 

分 类 号：X53[环境科学与工程—环境工程]