PEG化葛根素降低红细胞溶血的体内研究  

Study on Reducing Hemolysis by PEGylated Puerarin in Vivo

作　　者：刘新义[1] 李健和[1] 易利丹[1] 胡雄彬[1] 唐甜甜[1] 向大雄[1] 叶祖光[2] 

机构地区：[1]中南大学湘雅二医院药学部,长沙410013 [2]中国中医科学院,北京100700

出　　处：《中国现代应用药学》2014年第4期402-405,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：湖南省自然科学青年基金(14JJ3038)

摘　　要：目的探讨PEG修饰技术降低葛根素诱导红细胞溶血的体内研究。方法采用葛根素注射液和PEG化葛根素给予家兔每天等剂量静脉注射,连续7 d,每天取血3 mL,进行血细胞计数分析;7 d实验结束后再取血,检测红细胞渗透脆性和红细胞膜流动性。结果血细胞计数分析结果表明,PEG化葛根素组家兔的血细胞计数与空白对照组没有显著性差异,而葛根素注射液组家兔出现了血管内溶血现象;红细胞渗透脆性和红细胞膜流动性结果表明,PEG化葛根素可以降低红细胞渗透脆性和增强红细胞膜流动性。结论 PEG修饰技术可以减轻葛根素诱导血管内溶血的不良反应,更安全。OBJECTIVE To investigate the reducing hemolysis effect induced by PEGylation of puerarin via in vivo. METHODS Rabbits were once-daily given the same dose of puerarin injection and PEGylated puerarin intravenously for 7 days, 3 mL blood was taken every day and used for complete blood counts analysis. At the end of 7 days of experiment, blood was taken for the determination of osmotic fragility of RBC and erythrocyte membrane fluidity. RESULTS Blood counts analysis indicated that there was no significant difference between PEGylated puerarin group and blank control group. Osmotic fragility of RBC and erythrocyte membrane fluidity indicated that PEGylated puerarin could reduce osmotic fragility of RBC and enhance erythrocyte membrane fluidity. CONCLUSION PEGylation technology could reduce intravascular hemolysis induced by puerarin, which is more safe.

关 键 词：PEG化葛根素 葛根素注射液 血细胞计数分析 红细胞渗透脆性 红细胞膜流动性 

分 类 号：R965.2[医药卫生—药理学]