三种生防芽孢杆菌对5种烟田常用化合物的敏感性  

Sensitivities of three species of Bacillus to five common chemicals used in tobacco field

作　　者：汪汉成 刘圣明[2] 王进[3] 黄艳飞[4] 王茂胜 陆宁 李文红 

机构地区：[1]贵州省烟草科学研究院,贵阳550081 [2]河南科技大学林学院植物保护系,河南洛阳471003 [3]长江大学生命科学学院,湖北荆州434025 [4]长江大学农学院,湖北荆州434025 [5]贵州省植物保护研究所,贵阳550006

出　　处：《农药学学报》2014年第2期225-229,共5页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金(31360448);贵州省科学技术基金(黔科合J字[2011]2337号);中国烟草总公司贵州省公司科技项目(201305;201336;201436)

摘　　要：芽孢杆菌是植物病害生物防治中重要的生防细菌。采用浊度测定法研究了生防细菌——枯草芽孢杆菌、解淀粉芽孢杆菌及多粘芽孢杆菌对烤烟专用复合肥[m(N)∶m(P2O5)∶m(K2O)=10∶10∶25]、生石灰、甲霜灵、代森锰锌及硫酸链霉素5种烟草生产上常用化学品的敏感性。结果表明:甲霜灵对3种芽孢杆菌的生长无影响,而烤烟专用复合肥和生石灰对3种生防细菌均有一定的促生长作用;而代森锰锌和硫酸链霉素能显著抑制枯草芽孢杆菌、解淀粉芽孢杆菌和多粘芽孢杆菌的生长,其EC50值分别为14.15、11.88、11.30μg/mL和5.20、5.69和2.64μg/mL。Bacillus spp. is one type of the important biocontrol bacteria in agriculture. Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus polymyxa were three biocontrol bacteria against tobacco diseases, which were isolated from early study. Special compound fertilizer for tobacco [m（N） ： m（ P205 ） ： m（K20） = 10 ： 10 ： 25 ], calcium oxide, metalaxyl, mancozeb and streptomycin sulfate, which used frequently in tobacco field, against those three Bacillus species were evaluated. The results showed that metalaxyl had no effect on growth of B. subtilis, B. amyloliquefaciens and B. polymyxa, while compound fertilizer and calcium oxide could enhance the growth of the three bacteria. Mancozeb and streptomycin sulfate, however, significantly inhibited the growth of them, with ECs0values of 14. 15, 11.88 and 11.30 μg/mL, and 5.20, 5.69 and 2.64 μg/mL, respectively.

关 键 词：枯草芽孢杆菌 解淀粉芽孢杆菌 多粘芽孢杆菌 敏感性 甲霜灵 代森锰锌 硫酸链霉素 

分 类 号：S481[农业科学—农药学]