壳多糖抑制兔主动脉平滑肌细胞增殖机制的探讨  

Mechanism of chitosan inhibiting rabbit aortic smooth muscle cell proliferation

作　　者：王大新[1] 吴宗贵[1] 周斌[2] 姜远英[2] 殷明[2] 程明和[2] 

机构地区：[1]第二军医大学长征医院心血管内科,上海200003 [2]第二军医大学长征医院药学院药理学教研室

出　　处：《第二军医大学学报》2001年第2期159-160,共2页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金资助项目 !(3 9670 3 16)

摘　　要：目的 :探讨壳多糖抑制平滑肌细胞 (SMC)增殖的作用机制。方法 :通过随机引物法标记 c- m yc探针 ,应用 Northern印迹技术 ,观察壳多糖对小牛血清 (NBS)刺激的兔主动脉 SMC c- m yc m RNA表达的影响。结果 :当 NBS刺激血管 SMC 2 4h后 ,c- m yc m RNA表达较对照组明显增高 ;当壳多糖与 NBS合用时 ,c- m yc m RNA表达较 NBS组明显降低。结论 :壳多糖有可能通过抑制 NBS刺激的血管 SMC c- m yc m RNA的表达而达到抑制血管Objective: To study the mechanism of chitosan in inhibiting the proliferation of rabbit aortic smooth muscle cells(SMCs). Methods: By means of c myc probe labelled with random primers and Northern blot hybridization, we examined the effect of chitosan on vascular SMC c myc mRNA expression, which was stimulated by newborn bull serum (NBS,20%). Results: The oncogene c myc mRNA expression incerased in cultured vascular SMC 24 h after NBS exposure. These effects were inhibited by chitosan (20 μg/ml). Conclusion: Chitosan might inhibit the expression of vascular SMC c myc mRNA stimulated by NBS, through which the proliferation of vascular SMC are inhibited. [

关 键 词：壳多糖 主动脉平滑肌细胞 C-MYC 基因表达 细胞增殖 

分 类 号：R543.1[医药卫生—心血管疾病] O636.1[医药卫生—内科学]