积雪草总苷及其化学成分对瘢痕成纤维细胞增殖的抑制作用  

Inhibition against scar fibreblasts proliferation by the chemicals isolated from Centella asiatica

作　　者：欧阳丹薇[1] 邵燕[1] 孔德云[1] 张铮[2] 章一新[2] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院创新药物与制药工艺重点实验室,上海200040 [2]上海交通大学医学院附属第九人民医院整复外科,上海200010

出　　处：《世界临床药物》2014年第4期215-220,共6页World Clinical Drug

基　　金：上海市自然科学基金项目(11ZR1435300)

摘　　要：目的研究积雪草总苷及从中分离得到的主要单体化合物对瘢痕增生的抑制作用。方法以体外培养的人增生性瘢痕成纤维细胞为试验对象,经不同浓度的样品作用后,用MTT法测定样品对瘢痕成纤维细胞增殖能力的影响,用LDH法测定样品对细胞的毒性作用。结果 8个试验药物对瘢痕成纤维细胞增殖抑制作用由强到弱的顺序为:羟基积雪草酸>积雪草酸>羟基积雪草苷>centellasaponin B>积雪草总苷>积雪草苷、积雪草苷B>centelloside C。结论羟基积雪草酸、积雪草酸、羟基积雪草苷和centellasaponin B均可抑制瘢痕成纤维细胞的增殖,且作用强于积雪草总苷。Objective To observe the inhibition on proliferation of human hypertrophic scar fibroblasts by sapanins and triterpenoids isolated from Centella asiatica. Methods Culturing fibroblasts were treated with 8 test compound solution in different concentration. Their anti-proliferative capability was observed by MTT, and their toxicity was observed by LDH. Results The inhibition to scar fibroblast proliferation was ranged from strong to weak： madecassic acid^asiatic acid〉 madecassoside 〉 centellasaponin B 〉 total saponins 〉 asiaticoside, asiaticoside B 〉 centelloside C. Conclusion Madecassic acid, asiatic acid, madecassoside and centellasaponin B can inhibit the proliferative capability of the human hypertrophic scar fibroblasts, and are better than total saponin of Centella asiatica.

关 键 词：积雪草 化学成分 五环三萜皂苷 抗瘢痕增生 

分 类 号：R932[医药卫生—生药学] R64[医药卫生—药学]