带血供肌瓣作为骨形态发生蛋白载体修复骨缺损的实验研究  

Vascular muscle flap combined with bone morphogenetic protein for forming bone bridge to repair bone defect: experimental study

作　　者：裴国献[1] 杨润功[1] 魏宽海[1] 金丹[1] 

机构地区：[1]第一军医大学南方医院创伤骨科,广州510515

出　　处：《中华外科杂志》2001年第1期76-79,T003,共5页Chinese Journal of Surgery

基　　金：国家自然科学基金!资助项目 (39870 881)

摘　　要：目的　探讨带血供肌瓣作为骨形态发生蛋白 (BMP)载体修复骨缺损的可行性。　方法　观察带血供肌瓣复合BMP和单纯BMP组修复骨缺损时的成骨情况 ;对纤维蛋白粘合剂、带血供肌瓣、无血运肌瓣、同种异体脱钙骨 4种不同BMP载体的成骨能力进行比较。　结果　以指深屈肌支为蒂制备的带血供肌瓣复合BMP修复骨缺损 ,效果优于单纯BMP组 ;带血供肌瓣联合纤维蛋白粘合剂复合BMP组修复骨缺损 ,效果优于其它载体。　结论　带血供肌瓣可作为BMP的良好载体 ,带血供肌瓣联合纤维蛋白粘合剂作BMP的载体效果更优。Objective To investigate the effect of vascular muscle flap as a carrier of bone morphogenetic protein (BMP) to repair long bone defect. [WT5”HZ]Methods [WT5”BZ]Vascular muscle flap with BMP and BMP alone were implanted into the experimental models. Their conditions of new bone formation were observed and compared. Additionally, bone defects were divided into 4 groups in random and repaired respectively with the vascular muscle flap combined with FS/BMP (group A), vascular muscle flap/BMP (group B), bloodless muscle flap/BMP (group C), and autolyzed antigen-extracted allogeneic bone (AAA)/BMP (group D). Their abilities of bone forming were observed. [WT5”HZ]Results [WT5”BZ]In the group of vascular muscle flap combined with BMP, a large amount of cartilage was formed in the gaps of muscles by 3 weeks. The cartilage was absorbed and replaced by normal bone containing hematopoietic bone marrow by 6 weeks with obvious muscle cell atrophy. The wet bone weight of the new bone was (253 52±20 63) mg,which was significantly larger than that of the control group (172 22±13 95) mg ( P <0.01).In group A,the cartilage formed by 3 weeks and woven bone formed by 6 weeks;the haversion system formed and muscle cells disappeared by 9 weeks.Natural bone was found and the Tmax measured with torsion test was (0 82±0 04) N·m.The calcium content was (174 55±5 11) μg/g by 12 weeks.The ability of new bone formation in the 4 groups was in the following order: group A was similar to group D, group A>group B>group C. [WT5”HZ]Conclusions [WT5”BZ]Vascular muscle flap can serve as an effective carrier for BMP. Vascular muscle flap combined with FS as carrier is better than vascular muscle flap as a carrier alone.

关 键 词：骨形态发生蛋白质类 骨折愈合 动物实验 载体 带血供肌瓣 

分 类 号：R687.3[医药卫生—骨科学]