Reliable screening of pesticide residues in maternal and umbilical cord sera by gas chromatography-quadrupole time of flight mass spectrometry  

作　　者：FAN RuoJing ZHANG Fang WANG HaoYang ZHANG Li ZHANG Jing ZHANG Ying YU ChongTian GUO YinLong 

机构地区：[1]Shanghai Mass Spectrometry Center,Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences [2]Department of Gynaecology and Obstetrics, Yijishan Hospital of Wannan Medical College [3]Agilent Technologies (China) CO., Ltd.

出　　处：《Science China Chemistry》2014年第5期669-677,共9页中国科学（化学英文版）

基　　金：supported by the Science and Technology Commission of Shanghai Municipality(13ZR1448100)

摘　　要：The widespread use of pesticides induces heavy adverse effects on human health,especially for the pregnant women and the newborns.In this study,a screening method has been developed for the determination of multi-pesticides in maternal and umbilical cord sera.All pesticides in sera were collected using solid phase extraction(SPE),and analyzed by gas chromatography-quadrupole time of flight mass spectrometry(GC-QTOF MS).To set up the quality criteria,a database of 50 pesticides was created and the accurate masses of 3 up to 5 representative ions with their intensity ratios were included for each pesticide.In addition,a novel"identification points"(IPs)system relying on the accurate MS1 and MS2 spectra was used to interpret the data for each suspected pesticide.The methodology was then applied to a pair of maternal and umbilical cord sera.A total of six pesticide residues were screened out successfully.In conclusion,GC-QTOF MS combined with an accurate mass database seemed to be one of the most efficient tools for systematic pesticide analysis.The widespread use of pesticides induces heavy adverse effects on human health, especially for the pregnant women and the newborns. In this study, a screening method has been developed for the determination of multi-pesticides in maternal and um- bilical cord sera. All pesticides in sera were collected using solid phase extraction （SPE）, and analyzed by gas chromatog- raphy-quadrupole time of flight mass spectrometry （GC-QTOF MS）. To set up the quality criteria, a database of 50 pesticides was created and the accurate masses of 3 up to 5 representative ions with their intensity ratios were included for each pesticide. In addition, a novel ＂identification points＂ （IPs） system relying on the accurate MS1 and MS2 spectra was used to interpret the data for each suspected pesticide. The methodology was then applied to a pair of maternal and umbilical cord sera. A total of six pesticide residues were screened out successfully. In conclusion, GC-QTOF MS combined with an accurate mass database seemed to be one of the most efficient tools for systematic pesticide analysis.

关 键 词：pesticide residues SCREENING maternal and umbilical cord sera gas chromatography-quadrupole time of flight massspectrometry 

分 类 号：O657.63[理学—分析化学]