Combination of extraction tip and MALDI-TOF-MS for efficient separation and analysis of cysteine-containing peptides  

作　　者：WAN Dan CHEN Qi GAO MingXia ZHANG XiangMin YANG PengYuan 

机构地区：[1]Department of Chemistry and Institute of Biomedical Sciences, Fudan University

出　　处：《Science China Chemistry》2014年第5期703-707,共5页中国科学（化学英文版）

基　　金：financially supported by the National High-Tech R&D Program(2012AA020202);the National Basic Research Program of China(2012CB910604);the National Natural Science Foundation of China(21275034,21175026)

摘　　要：In our work, a new extraction tip with gold-modified polymer is developed. The simple, self-made and extremely economical tips were successfully applied to capture cysteine-containing peptides. The loading capacity of a tip （column bed： 0.3 mm di- ameter, 5 mm length） is 2-4 lag peptides. We can make one tip in 30 s and each costs less than 0.1 cent. The use of these tips can achieve a stable analysis with less background interference, even for 10 ng target peptides. Compared with other separation techniques, our method can save much time and energy while providing a means to selectively capture cysteine-containing peptides from complex analyte due to the strong interaction. All results showed that our new extraction tips have minimal cost and perfect selectivity; thus they have great potential in sample pretreatment systems for proteomics.In our work,a new extraction tip with gold-modified polymer is developed.The simple,self-made and extremely economical tips were successfully applied to capture cysteine-containing peptides.The loading capacity of a tip(column bed:0.3 mm diameter,5 mm length)is 2–4μg peptides.We can make one tip in 30 s and each costs less than 0.1 cent.The use of these tips can achieve a stable analysis with less background interference,even for 10 ng target peptides.Compared with other separation techniques,our method can save much time and energy while providing a means to selectively capture cysteine-containing peptides from complex analyte due to the strong interaction.All results showed that our new extraction tips have minimal cost and perfect selectivity;thus they have great potential in sample pretreatment systems for proteomics.

关 键 词：extraction tip matrix-assisted laser desorption/ionization time-of-flight mass spectrometry cysteine-containingpeptides 

分 类 号：O621.2[理学—有机化学]