左归丸对未成熟大鼠子宫发育的影响  

Effect of Zuo-Gui-Wan on Uterine Development in Immature Rats

作　　者：段恒[1] 周滢[1] 

机构地区：[1]重庆医科大学中医药学院,重庆400016

出　　处：《世界科学技术-中医药现代化》2014年第2期364-367,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：重庆医科大学校级课题(XBYB2008091):左归丸对大鼠生殖器官血管构筑和VEGF及其受体的影响;负责人:段恒

摘　　要：目的:探讨左归丸对大鼠子宫发育与血管生成的影响。方法:将40只SD大鼠,体质量(160~170)g,随机分为未成熟大鼠组(B组)、激素组(C组)、左归丸低剂量组(D组)、左归丸高剂量组(E组),另10只SD大鼠,体质量(220~250)g,作成熟大鼠组(A组)。A、B组分别灌服蒸馏水20 g·kg-1,C、D、E组分别灌服倍美力混悬液65μg·kg-1、左归丸浸膏(相当于生药11 g·kg-1)、左归丸浸膏(相当于生药33 g·kg-1),每天1次,连续15天。观察大鼠子宫组织形态学变化;采用酶免分析法测定血清雌二醇(E2)水平。另将SD大鼠(160~170)g随机分为未成熟大鼠组、激素组(65μg·kg-1)、左归丸组(相当于生药33 g·kg-1),SD大鼠(220~250)g作成熟大鼠组,每组6只。应用墨汁灌注法观察子宫肌层血管。结果:与A、B组比较,C^E组均可增加大鼠子宫内膜厚度,C、E组均可增加大鼠肌层厚度、被覆上皮高度、腺上皮高度、腺腔内径、腺体直径;与A、B组比较,C^E组均可明显提高大鼠血清E2水平(P<0.01或P<0.05)。激素组、左归丸组的子宫肌层血管密度和管腔明显增大(P<0.05)。与未成熟大鼠组比较,激素组、左归丸组均可增加大鼠子宫肌层大血管数及血管总数(P<0.05或P<0.01)。结论:左归丸可促进未成熟大鼠子宫发育,其作用与提高雌激素水平及促进血管生成,增加子宫局部血供有关。This article was aimed to study the effect of Zuo-Gui-Wan （ZGW） on uterine development and angiogen-esis in the rats. The SD rats （160-170）g were randomly divided into the immature rat group （group B）, steroid group （group C）, low-dose ZGW group （group D）, and high-dose ZGW group （group E）. And 10 SD rats （220-250） g were used as the mature rat group （group A）. There were 10 rats in each group. Intragastric administration of dis-tilled water （20 g·kg-1） was given to rats in group A and group B. Intragastric administration of conjugated estrogens suspension （65 μg·kg-1）, ZGW extract （which was equivalent to crude drug of 11 g·kg-1）, ZGW extract （which was equivalent to crude drug of 33 g·kg-1） was given to rats in group C, D and E, respectively. The medication was giv-en once a day, for 15 days. Uterine histomorphology of rats were observed. In addition, E2 level in blood were measured by enzyme immunoassay （EIA）. And rats （160-170）g were randomly divided into the immature rat group, steroid group （65 μg·kg-1）, ZGW group （which was equivalent to crude drug 33 g·kg-1）, and SD rat （220-250）g as mature group, with 6 rats in each group. Uterine micrangium was measured by infusion with ink. The results showed that compared with group A and B, group C to group E can all increase the thickness of endometrium of rat＆#39;s uterus. Group E and C can increase myometrial thickness, covering epithelium height, glandular epithelium height, glandular cavity diameter, and glandular diameter. Compared with group A and B, group C to group E can obviously increase E2 level in blood of rats （P〈 0.01 or P〈 0.05）. In the steroid group and ZGW group, the vascular density and diameter in the myometrium were obviously increased. Compared with the immature rat group, the steroid group and ZGW group can increase the amount of large blood vessels and total blood vessels in the myometrium （P〈 0.05 or P〈 0.01）. It was concluded that ZGW can improve ut

关 键 词：左归丸 大鼠 子宫 血管生成 

分 类 号：R285.5[医药卫生—中药学]