补气活血类中药对血管内皮细胞增殖的影响  

Influence on Proliferation of Vascular Endothelial Cells by Qi-reinforcing and Blood-activation Chinese Medicine

作　　者：李冰冰[1] 唐德才[1] 臧文华[1] 

机构地区：[1]南京中医药大学基础医学院,南京210046

出　　处：《世界科学技术-中医药现代化》2014年第3期572-577,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(81073072):基于活体成像技术的补气活血药配伍抗卵巢癌转移时效关系研究;负责人:唐德才;国家自然基金委面上项目(81373990):黄芪;莪术配伍调控肝癌血管生成的体视学及分子机制研究;负责人:唐德才;江苏省人力资源和社会保障厅六大人才高峰项目(013034010004):基于SDF-1/CXCR4通路的补气活血药配伍对血管生成的调控规律研究;负责人:唐德才

摘　　要：目的:观察补气活血类中药含药血清对体外培养的人脐静脉血管内皮细胞(HUVEC)增殖的影响,并初步筛选出对HUVEC增殖能力具有较明显促进、抑制作用的药物。方法:建立HUVEC的体外培养的细胞模型,采用血清药理学方法,将10种活血化瘀药分别与3种补气药按照1∶2、1∶1、2∶1三种不同的配比两两配伍组合,组成90对补气活血药药对,制备90对补气活血药药对和10种活血化瘀药的含药血清。应用MTT比色法观察不同活血化瘀药和补气活血药药对的含药血清对HUVEC增殖能力的影响。结果:与空白血清组相比较,大部分的活血化瘀药、补气活血药对含药血清对HUVEC的增殖能力都有较为显著的影响(促进或者抑制),其中丹参组、川芎组、赤芍组,丹参黄芪(1∶2)组、丹参黄芪(1∶1)组、川芎黄芪(1∶2)组可明显促进HUVEC增殖能力(P<0.05或P<0.01);三棱组、莪术组、丹皮组、莪术黄芪(2∶1)组、三棱人参(2∶1)组、莪术黄芪(1∶1)组可明显抑制HUVEC增殖(P<0.05或P<0.01)。结论:不同的活血化瘀药和补气活血药配伍组成对HUVEC的增殖能力具有不同的促进或抑制作用,这可能为这些药物在细胞层面上促进或抑制血管生成的作用机制。This study was aimed to observe the influence of in v itro proliferation ability of human umbilical vein endothelial cell （HUVEC） by qi-reinforcing and blood-activation Chinese medicine and preliminary screen the medicals with obvious promoting or inhibiting effects on proliferation ability of HUVEC. The in vitro HUVEC culture model was established. Serum pharmacological method was used. The 10 kinds of blood-activation stasis-removing herbs and 3 kinds of qi-reinforcing herbs were combined according to the proportion of 1：2, 1：1, and 2：1. There were 90 couplet medicines of qi-reinforcing and blood-activation. Then, 90 couplet medicines of qi-reinforcing and blood-activation and 10 kinds of blood-activation and stasis-removing herbs were prepared. MTT method was used to detect the effect of the medicated serum of these couplet medicines of blood-activation and stasis-removing herbs or qi-reinforcing and blood-activation herbs on the proliferation ability of HUVEC. The results showed that compared with the blank control serum group, most of the medicated serum of blood-activation and stasis-removing herbs or qi-reinforcing and blood-activation herbs had relatively obvious influence （promoting or inhibiting） on the proliferation ability of HUVEC. Among them, the Salvia group, Ligusticum Chuanxiong group, Paeonia group, Salvia-A stragalus （1：2） group, Salvia-A stragalus （1：1） group, and Ligusticum Chuanxiong-A stragalus （1：2） group had significant promoting effect on the proliferation ability of HUVEC （P 〈 0.05 or P 〈 0.01）; the Sparganium group, Curcuma zedoary group, Cortex moutan group, Curcuma zedoary-A stragalus （2：1） group, Sparganium-Ginseng （2：1） group, Curcuma zedoary-A stragalus （1：1） group had significant inhibiting effect on the proliferation ability of HUVEC （P 〈 0.05 or P 〈 0.01）. It was concluded that different compatibility of Chinese medicines of blood-activation and stasis-removing herbs or q i-reinforcing and blood-activation herbs

关 键 词：补气活血药 血管内皮细胞 增殖 四唑盐比色法 

分 类 号：R285.5[医药卫生—中药学]