风药生姜增效下瘀血汤对肝纤维化大鼠HPC-Ⅲ、ColⅥ、LN的影响  

Effects of Xia-Yu-Xue Decoction with Ginger on HPC-Ⅲ,ColⅥ and LN in Hepatic Fibrosis Rat Serum

作　　者：刘然[1] 罗再琼[1] 何利黎[1] 张天娥[1] 

机构地区：[1]成都中医药大学基础医学院,成都610015

出　　处：《世界科学技术-中医药现代化》2014年第3期587-590,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(81072737):基于TGF-β/Smad通路研究"风药"对活血化瘀增效的作用机制;负责人:罗再琼

摘　　要：目的:通过风药生姜配伍下瘀血汤对肝纤维化的抑制,揭示风药生姜增强活血化瘀的作用机制。方法:用50%CCl4橄榄油溶液皮下注射建立大鼠肝纤维化模型,8周造模成功后,将大鼠随机分为正常对照组(A组)、模型对照组(B组)、下瘀血汤组(C组)、生姜低剂量配伍下瘀血汤组(D组),生姜中剂量配伍下瘀血汤组(E组)。继续造模的同时并用药物干预至12周,观察各组药物治疗后的一般情况和血清肝纤维指标变化及肝组织学变化。结果:与模型组比较,下瘀血汤组和风药组大鼠血清Ⅲ型前胶原(HPC-Ⅲ)、Ⅵ型胶原(ColⅥ)、层粘连蛋白(LN)含量明显降低(P<0.05或P<0.01),且生姜配伍下瘀血汤组上述指标较单纯下瘀血汤组明显降低(P<0.05)。结论:风药生姜配伍下瘀血汤可增加活血化瘀药的作用,为中医药抗肝纤维化提供新的用药配伍思路。This article was aimed to study the inhibition of hepatic fibrosis by X ia-Y u-X ue （XYX） decoction with wind medicine ginger, in order to reveal the action mechanism of wind medicine ginger in increasing blood-activation and stasis-removing. The hepatic fibrosis rat models were established with 50% CCl4 hypodermic injection for 8 weeks. Rats were randomly divided into the normal control group （group A）, model control group （group B）, XYX decoction group （group C）, XYX decoction with low-dose ginger group （group D）, and the XYX decoction with medium-dose ginger group（group E）. During the model establishment, medications were intervened until the 12th week. Observation was given on the general body status, changes of serum hepatic fibrosis indexes, and hepatic histological changes after medication in each group. The results showed that compared with the model group, contents of HPC-Ⅲ, ColⅥand LN in rat serum were obviously reduced in the XYX decoction group and wind medicine group （P〈 0.05 or P〈0.01）. And indexes mentioned above in the XYX decoction combined with ginger group were lower than the XYX decoction group（P〈 0.05）. It was concluded that XYX decoction with wind medicine ginger can increase the blood-activation and stasis-removing effect, which provided new way of medication compatibility in the Chinese medicine treatment of hepatic fibrosis.

关 键 词：生姜 肝纤维化 风药 活血化瘀 

分 类 号：R285.5[医药卫生—中药学]