LC-UV法测定华中五味子中五味子酯甲、五味子甲素、五味子乙素含量  

Simultaneous determination of schisantherin A,deoxyschizandrin andγ-schisandrin in S. sphenanthera Rehd.et Wils.by LC-UV

作　　者：汪荣斌[1,2] 王义祁[1] 秦亚东[1] 周宙[1] 王存琴[3] 李林华 刘晓龙[1,2] 黄浩[1,4] 

机构地区：[1]安徽中医药高等专科学校,安徽芜湖241000 [2]安徽中药资源研究所,安徽芜湖241000 [3]皖南医学院,安徽芜湖241000 [4]北京同仁堂安徽中药材有限公司,安徽铜陵244000

出　　处：《长春中医药大学学报》2014年第2期213-215,共3页Journal of Changchun University of Chinese Medicine

基　　金：安徽芜湖市科技计划2009年度重点科技项目(医药7-2);安徽省高职高专院校专业带头人(皖教秘人[2011]2号)

摘　　要：目的建立同时测定五味子酯甲、五味子甲素、五味子乙素的LC-UV含量测定方法。综合考察皖南山区野生华中五味子的内在品质,为该品种转为栽培品提供理论依据。方法采用LC-UV法,Agilent TC-C18(2)色谱柱(250 mm×4.6 mm I.D,5 um),以甲醇-水为流动相,梯度洗脱,流速为1 mL/min,柱温30℃,检测波长为240 nm,测定五味子酯甲、五味子甲素、五味子乙素。结果皖南山区野生华中五味子中五味子酯甲、五味子甲素、五味子乙素平均含量分别为0.24%、0.46%、1.22%。结论皖南山区野生华中五味子中五味子酯甲平均含量为0.24%,符合《中国药典》2010版的要求:五味子酯甲含量不低于0.20%,可供药用。通过三指标的综合控制,为皖南华中五味子的品质评价和栽培提供理论支持。Objective To investigate the determination of the contents of Schisantherin A, Deoxyschizandrin and γ-schizan- drin in S. sphenanthera Rehd. et Wils. by LC-UV and explore the quality of wild S. sphenanthera Rehd. etWils, in Wannan mountainous area to provide a reliable measure for quality control of GAP. Methods 1. LC-UV method was established with a Agilent TC-C18（2） column （5 μm,4.6 × 250 mm）, a mobile phase of Methanol-Water solution a flow rate of 1.0 mL· min- 1 and a detection wavelength of 240 nm in order to determine the contents of Schisantherain A, Deoxyschizandrin and γ- schizandrin. The column temperature was maintained at 30 ℃. Results The average contents of Schisantherain A, De- oxyschizandrin andγ-schizandrin in the decoction of wild S. sphenanthera Rehd. et Wils. in Wannan mountainous area were 0.24% ,0.46%, 1.22% respectively. Conclusion The average content of Schisantherin A in wild S. sphenanthera Rehd.et Wils in Wannan mountainous area was 0.24% which is in accordance with the requirements Chinese Pharmacopoeia （2010 Edition）. The content of Schisantherin A is no less than 0.20 %, which can be used for medicine. By the comprehen- sive control of three indicators, theoretical support for the quality evaluation and cultivation of S. sphenanthera Rehd. et Wils. in southern Anhui can be provided.

关 键 词：华中五味子 皖南山区 含量测定 LC-UV 

分 类 号：R284.1[医药卫生—中药学]