TGFβ_1对人血管内皮细胞整合素β_3及粘着斑激酶表达的影响  

Effects of Transforming Growth Factor β_1 on the Expression of Integrin β_ 3 and the Activity of Focal Adhesion Kinase on Human Vascular E ndothelial Cell

作　　者：傅华[1] 刘小菁[2] 杨丽[3] 强鸥[2] 黄明慧[2] 

机构地区：[1]华西医科大学附属第一医院心内科,成都610041 [2]华西医科大学附属第一医院内科实验室,成都610041 [3]华西医科大学附属第一医院消化内科,成都610041

出　　处：《华西医科大学学报》2001年第1期21-23,共3页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金资助项目! (批准号 3970 0 0 6 8

摘　　要：目的　观察转化生长因子 β1 ( TGFβ1 )对培养的人血管内皮细胞表面整合素 β3 表达及粘着斑激酶 ( focal adhesion kinase,FAK)活性的影响。方法　采用细胞 - EL ISA和免疫沉淀 -蛋白质酪氨酸激酶活性测定法。结果　 1ng/ m l、5 ng/ ml及 10 ng/ ml浓度的 TGFβ1 分别作用于培养的人血管内皮细胞 ( ECV30 4) 6、2 4和 48小时后 ,其细胞表面整合素 β3 蛋白的表达均增加 ( P<0 .0 5 ) ,且呈剂量依赖性。 5 ng/ m l和 10 ng/ m l的 TGFβ1 作用于培养的 ECV30 46小时和 2 4小时后 ,细胞中 FAK活性明显增高 ( P<0 .0 5 )。结论　 TGFβ1 促进血管内皮细胞表面整合素 β3 的表达和 FAK活性增高 ,可能是单核细胞和内皮细胞之间粘附功能增加 。Objective To observe the effects of transforming growth factors β 1 (TGFβ 1) on the expression of integrin β 3 and the acti vity of focal adhesion kinase (FAK). Methods This study was pe rformed on cultured human endothelial cells (EC) by using cell ELISA and immuno precipitation tyrosine kinase assay respectively. Results Und er the stimulus of TGFβ 1, there was a dose dependent increase in the expre ss ion of integrin β 3 chain in the surface of EC. And after the cultured EC were treated with 5ng/ml or 10ng/ml TGFβ 1 for 6 or 24 hours, the FAK activity in EC increased significantly as compared with the control group ( P <0.05). Conclusion The expression of integrin β 3 and the activity of F AK on EC were regulated by TGFβ 1, and this regulation may be important in cel l adherence, angiogenesis, and in the pathophysiology of atherosclerosis.

关 键 词：血管内皮细胞 转化生长因子Β 整合素Β3 粘着斑激酶 动脉粥样硬化 

分 类 号：R543.12[医药卫生—心血管疾病]