绵羊7号染色体46843356位点多态性与尾脂沉积相关性研究  

Relationship between the polymorphsim of 46843356 locus on the 7th chromosome and tail fat deposition

作　　者：张伟[1,2,3] 蒋曙光[4] 沈敏[1] 赵伟利 许瑞霞[1,3] 宋广超[1,3] 刘守仁[1] 王新华[1] 甘尚权[1] 

机构地区：[1]新疆农垦科学院畜牧兽医研究所,新疆石河子832000 [2]新疆农业职业技术学院动物科技学院,新疆昌吉831100 [3]石河子大学动物科技学院,新疆石河子832003 [4]新疆维吾尔自治区畜牧总站,新疆乌鲁木齐830000

出　　处：《畜牧与兽医》2014年第4期25-29,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家973前期专项(2011CB111501);国家自然科学基金委-新疆联合重点项目(U1130302)

摘　　要：为探讨绵羊7号染色体上46843356位点多态性与绵羊尾脂沉积的关系,以尾型极端差异的阿勒泰羊、小尾寒羊、中国美利奴细毛羊以及萨福克羊为研究对象,利用PCR-RFLP检测该位点在群体中的多态性。结果表明:46843356位点在尾脂沉积能力较差的中国美利奴细毛羊和萨福克羊群体以AA基因型为主,而尾脂沉积能力强的阿勒泰羊群体则以GG和GA基因型为主,阿勒泰羊和小尾寒羊G/A等位基因频率比值为2.48和4.22,分别是萨福克羊的83倍和141倍。卡方检验结果表明,阿勒泰羊和小尾寒羊在该位点上处于Hardy-Weinberg平衡状态(P>0.05),而中国美利奴细毛羊和萨福克羊在该位点上处于Hardy-Weinberg极度不平衡状态(P<0.01)。以上结果提示,绵羊7号染色体上46843356SNP位点在脂尾(臀)与瘦尾绵羊群体中存在较大差异,可作为理想的分子标记应用于高、低脂绵羊品种选育。To investigate the relationship between the fat accumulation and polymorphsim of 46843356 locus on 7th chromosome in sheep, the present study was conducted by using the Ahay, Small Tail Han, Chinese Merino and Suffolk sheep, extremely difference on tail types,through PCR-RFLP methods. The result showed that the AA genotype of 46843356 locus was preponderant genotype in the Chinese Merino and Soffolk sheep which were thin-tail sheep breed, and the GG and GA genotype in the Small Tail Han and Ahay sheep which were fat-tail or fat-rump sheep breed. The result of ehi-square analysis suggested that the allele frequencies and genotype frequencies were in Hardy- Weinberg equilibrium （ P〉0. 05 ） at this locus in Small Tail Han and Ahay sheep, but not in Hardy-Weinberg equilibrium in Chinese Merino and Suffolk sheep （P〈0. 01 ）. A great difference were found between the fan-tail and thin-tail sheep on 46843356 locus of the 7th chromo- some, implying a perfect moleculer marker which could be used in high or low fat sheep breeding.

关 键 词：脂尾性状 阿勒泰羊 多态性 基因型 

分 类 号：S826.8[农业科学—畜牧学]