胎盘生长因子与妊娠高血压综合征发病的相关性研究  

Relationship between placenta growth factor and the pathogenesis of pregnancy induced hypertension syndrome

作　　者：刘伟[1] 林其德[1] 王立东 陈珠萍[1] 

机构地区：[1]上海第二医科大学附属仁济医院妇产科,200001 [2]上海免疫研究所

出　　处：《中华妇产科杂志》2001年第1期5-8,T001,共5页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　探讨胎盘生长因子 (PLGF)与妊娠高血压综合征 (妊高征 )发病的关系。方法用酶联免疫吸附法 (ELISA)、免疫组织化学染色法和逆转录 聚合酶链反应法 (RT PCR)检测 2 3例妊高征患者 (妊高征组 )、2 0例正常妊娠妇女 (正常妊娠组 )的外周血及其新生儿脐静脉血PLGF水平、胎盘蜕膜PLGF蛋白及其mRNA表达。结果　 (1)妊高征组外周血PLGF水平 [(112 7± 6 3 8) μg/L]低于正常妊娠组 [(2 0 0 3± 140 9) μg/L],两组比较 ,差异有显著性 (P <0 0 5 ) ,且与新生儿体重和胎盘重量呈正相关 ,相关系数分别为 0 4、0 6。 (2 )妊高征组合体滋养细胞、绒毛间质和蜕膜PLGF表达均显著低于正常妊娠组 (P <0 0 1,P <0 0 1,P <0 0 5 ) ,其合体滋养细胞PLGF表达水平与妊高征病情的轻、重有关。 (3)妊高征组胎盘和蜕膜组织总PLGFmRNA表达均显著低于正常妊娠组 (P <0 0 5 ,P<0 0 5 ) ,尤其胎盘组织主要表现为PLGF 2mRNA表达明显下降 (P <0 0 1)。Objective To determine whether pregnancy induced hypertension syndrome (PIHs) is associated with placenta growth factor (PLGF). Methods PLGF level in the maternal and umbilical venous serum collected from 23 PIHs patients and 20 normotensive controls was measured by enzyme linked immunoassay (ELISA), and expression of PLGF and its mRNA in the placenta and decidua from them was assessed by the immuno histochemistry and reverse transcriptional polymerase chain reaction (RT PCR). Results (1) PLGF levels in the maternal serum from PIHs [(112 7±63 8) μg/L] were significantly lower than those from normal pregnancies [(200 3±140 9) μg/L], ( P <0 05), and they were correlated with infant birth weight and placental mass ( r =0 4, r =0 6). (2) Expression of PLGF in sycytiotrophoblast and villous mesenchyme and decidua from PIHs significantly decreased respectively ( P <0 01, P <0 01, P <0 05), and expression of PLGF in sycytiotrophoblast was associated with the severity of PIHs ( P <0 05). (3) The transcription of total PLGF mRNA in the placenta and decidua from PIHs decreased significantly ( P <0 05 and P <0 05 respectively). Particularly, the transcription of PLGF 2 mRNA decreased significantly in the placenta from PIHs. Conclusion The abnormal expression of PLGF may play an important role in the pathogenesis of placental ischaemia hypoxia in PIHs.

关 键 词：妊娠高血压综合征 ELISA 胎盘生长因子 PLGF RT-PCR 免疫组织化学 

分 类 号：R714.246[医药卫生—妇产科学]