HPLC法同时测定依普罗沙坦/氢氯噻嗪片的两组分含量  

Simultaneous Determination of Eprosartan /Hydrochlorothiazide Tablets by HPLC

作　　者：李旸[1] 谢向阳[1,2] 陈晨[1] 韩亮[1] 廖祥茹[1] 陈鹰[1] 

机构地区：[1]广州军区武汉总医院,武汉430070 [2]军事医学科学院毒物药物研究所

出　　处：《中国药师》2014年第4期582-584,共3页China Pharmacist

摘　　要：目的：建立 HPLC 法测定复方依普罗沙坦/氢氯噻嗪片含量的方法。方法：采用菲罗门 C18色谱柱（250 mm×4.6 mm,5μm）,以0.5%甲酸水溶液-乙腈（60∶40, pH 2.80）为流动相,流速1.0 ml·;min-1,检测波长272 nm,柱温30℃,进样量20μl。结果：依普罗沙坦质量浓度在60.0~1200.0 mg·;L-1范围内（r=0.9999）,氢氯噻嗪质量浓度在1.25~25.00 mg·; L-1范围内与峰面积呈良好的线性关系（r=0.9999）,平均回收率分别为100.02%（RSD=0.35%,n=9）、97.93%（RSD=1.54%, n=9）。结论：本方法简便、快速、准确,重复性好,可用于复方依普罗沙坦/氢氯噻嗪片中两组分的含量测定。Objective：To establish an HPLC method for the simultaneous determination of eprosartan/hydrochlorothiazide tablets. Methods：Isocratic separation was achieved on a Phenomenex C18 column（250 mm × 4. 6 mm, 5 μm） using the mobile phase com-posed of 0. 5% formic acid-acetonitrile（60∶40, pH 2. 80）. The flow rate was 1. 0 ml·min-1, the detection wavelength was 272nm, the column temperature was 30℃ and the injection volume was 20μl. Results：The linearity between peak area and concentration was observed within the range of 60. 0-1 200. 0 mg·L-1（r=0. 999 9） for eprosartan and 1. 25-25. 00 mg·L-1（r=0. 999 9） for hydro-chlorothiazide. The mean recovery of eprosartan and hydrochlorothiazide was 100. 02%（RSD=0. 35%, n=9） and 97. 93%（RSD=1. 54%, n=9）, respectively. Conclusion：The method is simple, sensitive and accurate, and can be applied in the determination of eprosartan/hydrochlorothiazide tablets.

关 键 词：依普罗沙坦 氢氯噻嗪 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]