润肺治纤汤对肺纤维化大鼠肺组织形态学变化的影响  

作　　者：甘卉[1] 王忠超[1] 李伟[1] 宋晓环 刘诗音[1] 朱莉[1] 胡金秋[1] 王岚[1] 

机构地区：[1]长春医学高等专科学校,吉林130031

出　　处：《当代医学》2014年第11期17-18,共2页Contemporary Medicine

基　　金：吉林省教育厅"十二五"科研项目[2012]第417号

摘　　要：目的探讨润肺治纤汤对实验性肺纤维化大鼠的治疗作用。方法 Wistar大鼠均分为正常组、模型组、治疗组(n=8)。运用大鼠气管内滴入博来霉素的方法复制肺纤维化动物模型,造模后治疗组每天4 mL润肺治纤汤灌胃,正常组、模型组每日等量生理盐水灌胃。给药28 d后检测肺组织羟脯氨酸(HYP)含量,肺组织苏木精-依红(HE)染色观察病理变化,并做肺组织形态学定量分析。结果模型组肺组织HYP含量较正常组显著增加(P<0.01),肺组织纤维化显著,肺泡间隔厚度较正常组显著增加(P<0.01);治疗组肺HYP含量与模型组比较显著降低(P<0.05),肺组织纤维化程度轻于模型组,肺泡间隔和肺泡间隔核选区面积与模型组比较有统计学意义(P<0.01)。结论润肺治纤汤具有减轻实验性肺纤维化大鼠肺纤维化的作用。Objective To explore the therapeutic effect of Moistening Lung Treating Fibrosis Soup on rats models of experimental pulmonary fibrosis. Methods Wistar rats were divided into normal group, model group, treatment group with 8 rats in each group. Using rat tracheal instillation of bleomycin to copy pulmonary fibrosis animal model. After modeling, the treatment group daily 4 mL Moistening Lung Treating Fibrosis Soup decoction, the normal group and model group normal saline decoction. （intratracheal instillation of bleomycin）Lung hydroxyproline （HYP） content detected,the pathological changes of lung tissue was observed by using hematoxylin eosin （HE） staining after administered 28 days and quantitative morphological analysis was done. Results The content of HYP in lung tissue and alveolar septum thickness of model group was significantly increased than normal group （P〈0.01）. HYP content and degree of pulmonary fibrosis in treatment group was obviously lower than model group （P〈0.05）. Alveolar septa and alveolar septal nucleus constituency area compared with the model group was statistically significant （P〈0.01）. Conclusion Moistening Lung Treating Fibrosis Soup can reduce pulmonary fibrosis for experimental pulmonary fibrosis rats .

关 键 词：肺纤维化 大鼠 病理 形态定量分析 

分 类 号：R285.5[医药卫生—中药学]