GABA_A受体在爪蟾卵母细胞的表达  

Brain GABA_A receptors expression in Xenopus oocytes in rats

作　　者：张献全[1] 钱桂生[1] 顾全宝[2] 赵剑星[2] 

机构地区：[1]第三军医大学附属新桥医院全军呼吸内科研究所,重庆400037 [2]中国科学院上海细胞生物学研究所,上海200031

出　　处：《第三军医大学学报》2001年第2期149-150,共2页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目!( 39570 30 6 )

摘　　要：目的　将鼠源γ 氨基丁酸A型 (GABAA)受体表达于爪蟾卵母细胞膜上并行功能测定。方法　微量注射仪将纯化的大鼠脑mRNA注入准备好的爪蟾卵母细胞内并于 19℃温浴 48h以上使其表达 ;使用常规双电极电压钳记录卵母细胞表达的受体特性。结果　爪蟾卵母细胞注射大鼠脑mRNA 48h后有GABAA 受体的表达 ,此受体具有天然GABAA 受体的氯通道特性。结论　爪蟾卵母细胞具有表达外源GABAA 受体的特性 ,是研究GABAA 受体生理和药理功能的一种重要工具。Objective To express GABA A receptors in Xenopus oocytes. Methods mRNA was isolated from brain of neonatal rat and injected into prepared Xenopus oocytes. Voltageclamp was used to measure electrophysiological character of GABA A receptors expressed in oocytes. Results GABA A receptors were expressed in Xenopus oocytes 48 h after injection at 19℃ and the Cl - channel character was similar to those of native's receptors. Conclusion Xenopus oocyte can be used to express exogenous GABA A receptors, and is an important tool in studying physiological and pharmacological function of GABA A receptor subunits.

关 键 词：Γ-氨基丁酸受体 爪蟾卵母细胞 中枢神经系统 MRNA 

分 类 号：R338[医药卫生—人体生理学]