缺氧复合梭曼中毒PC_(12)细胞中[Ca^(2+)]、cAMP、CaM和Ca_(2+)/CaM-PKⅡ的变化  

Concentration of Ca^(2+) , contents of cAMP, CaM and Ca^(2+) /CaM-PK II in pheochro mocytoma PC_(12) cells after combined soman and hypoxia injury

作　　者：赵吉清[1] 吴强[1] 王仕丽[1] 魏相德[1] 董兆君[1] 李云鹏[1] 刘勇[1] 

机构地区：[1]第三军医大学预防医学系军事毒理学教研室,重庆400038

出　　处：《第三军医大学学报》2001年第2期169-171,共3页Journal of Third Military Medical University

基　　金：全军"九五"医学科研规划指令性课题!( 96L0 15)

摘　　要：目的　观察缺氧复合梭曼中毒后大鼠肾上腺嗜铬细胞瘤PC12 细胞内游离钙的浓度 ([Ca2 + ] )、cAMP、钙调蛋白 (CaM)和钙调蛋白激酶Ⅱ (Ca2 + /CaM PKⅡ )的变化。方法　采用放免技术 ,从细胞水平进一步观察缺氧复合梭曼中毒对PC12 细胞的毒性、细胞内 [Ca2 + ]变化、cAMP、CaM和Ca2 + /CaM PKⅡ的变化。结果　缺氧中毒组PC12 细胞cAMP、CaM含量在中毒后 2 4h明显高于单纯中毒组、缺氧对照组和正常对照组 ;缺氧中毒组PC12 细胞Ca2 + /CaM PKⅡ活性在中毒后 2 4h明显低于单纯中毒组、缺氧对照组和正常对照组。结论　 [Ca2 + ]、cAMP、CaM和Ca2 + /CaM PKⅡ在缺氧复合梭曼中毒PC12 细胞损伤机制中起了重要的作用。Objective To observe the changes of the concentration of Ca 2+ , contents of cAMP, CaM and activity of Ca 2+ /CaM PK II in pheochromocytoma PC 12 cells after combined soman and hypoxia injury. Methods The changes of [Ca 2+ ], and activity of CaM, cAMP and Ca 2+ /CaM PK II in PC 12 cells were studied after combined soman and hypoxia injury with radioimmunoassay. Results The changes of [Ca 2+ ], the contents of CaM, cAMP were significantly higher in hypoxic and soman intoxicated group than in soman intoxicated group and control group under hypoxia; but the activity of Ca 2+ /CaM PK Ⅱ were significantly decreased. Conclusion [Ca 2+ ], CaM, cAMP and Ca 2+ /CaM PK Ⅱ exert important role in the damage of PC 12 after combined soman and hypoxia injury.

关 键 词：缺氧 复合梭曼中毒 PC12细胞 钙离子 CAMP CAM CAM-PKⅡ 

分 类 号：R594.306[医药卫生—内科学] R827.122[医药卫生—临床医学]