体外不同强度脉冲压力刺激对兔桡骨缺损修复的影响  

Stimulation effects of pulsating pressures of different intensities in vitro on repair of rabbit radial defects

作　　者：户刚[1] 何树[1] 毕龙[1] 刘建[1] 王春梅[1] 李丹[1] 吕荣[1] 王军[1] 段春光[1] 孟国林[1] 吴拮[1] 李静[1] 魏琳岚[2] 

机构地区：[1]第四军医大学西京医院骨科,西安710032 [2]西安卫星测控中心教导大队

出　　处：《中华创伤骨科杂志》2014年第4期324-328,共5页Chinese Journal of Orthopaedic Trauma

基　　金：国家自然科学基金（81371982）

摘　　要：目的比较不同强度脉冲压力刺激对兔桡骨缺损修复的影响，为选取合适的压力刺激强度提供实验依据。方法取新西兰大白兔32只，构建5mm桡骨缺损模型，随机分为4组（n=8）：对照组术后正常喂养，不进行体外脉冲压力刺激；实验组于术后第4天分别给予不同强度的体外脉冲压力刺激持续至术后4周，1次／d，30min／次。脉冲频率为15Hz，并按照压力强度分为15—7kPa组，8-10kPa组，11～13kPa组。术后4周取材行标本大体观察、X线摄片和组织学观察以评价缺损修复情况。结果术后4周标本大体观察：8—10kPa组桡骨缺损处新生骨痂较其他3组明显偏多。术后4周x线片示4组骨缺损区均被大量高密度影填充，8—10kPa组已基本形成完全骨性连接，其他3组骨缺损区仍可见较多低密度间隙。术后2、4周x线片．dicom原始数据分析显示8—10kPa组骨缺损区x线灰度值最高，与其他3组比较差异有统计学意义（P〈0．05）。术后4周Lane-SandhuX射线评分8-10kPa组最高，与其他3组比较差异有统计学意义（P〈0．05）。术后4周VanGieson染色结果显示：8—10kPa组骨缺损区已有大量新生小梁骨完全填充，骨缺损两断端已基本形成完全骨性连接，其他3组骨缺损区有较多新生骨，其间尚存在许多纤维软骨团，骨缺损两断端未形成完全骨性连接。结论体外脉冲压力在8。10kPa范围内对骨缺损修复有明显的促进作用。Objective To determine an appropriate intensity of pulsating pressure by comparing the stimulation effects of pulsating pressures of different intensities on the repair of rabbit radial defects. Methods Animal models of radial defect of 5 mm in length, which had been created in a total of 32 New Zealand rabbits, were randomized into 4 groups （ n = 8） . The defects were repaired naturally in the control group （group A） while the other 3 experiment groups received stimulation of pulsating pressures of different intensities which started on the 4th day after surgery and continued every day for 4 weeks. The stimulation lasted 30 minutes each time and the frequency of pulsating pressure was 15 Hz. The 3 experimental groups were divided according to the intensities of pulsating pressure： 5 to 7 kPa （group B）, 8 to 10 kPa （group C） and 11 to 13 kPa （group D）. New bone formation in the 4 groups was evaluated by gross observation, X-ray radiography and Van Gieson staining at 4 weeks after surgery. Results By gross observation of the specimens, there was more new bony callus in the defects in group C than in the other 3 groups. X-ray ra- diography showed the bone defects in each group were filled with massive high-density shadow; both ends of the bone defects in group C were almost connected completely by the newly formed bone while quite a number of low-density gaps were observed in the defect area in the other 3 groups. The mean Lane-Sandhu X-ray score was the highest in group C, significantly higher than those in the other 3 groups （ P 〈0. 05）. Analysis of the X-ray grayscale values of. dicom data showed that the X-ray grayscale value in group C was significantly higher than those in the other 3 groups （ P 〈 0. 05）. Van Gieson staining observed that the defects in group C were filled with a large amount of newly-formed trabecular bone and both ends of bone defects were almost connected completely by the trabocular bone, but that although a large amount of new bone was observed in the de

关 键 词：桡骨骨折 物理治疗技术 压力 兔 

分 类 号：R687.3[医药卫生—骨科学]