异搏定在离体肾保存中应用的实验研究  

Experimental study of Verapamil in kidney graft preservation

作　　者：范明齐[1] 张银甫[1] 莫华根[1] 黄赤兵[1] 严治林[1] 王平贤[1] 

机构地区：[1]第三军医大学附属新桥医院泌尿外科,重庆400037

出　　处：《第三军医大学学报》2001年第2期215-216,共2页Journal of Third Military Medical University

摘　　要：目的　研究钙拮抗剂异搏定在离体肾保存中对肾细胞的保护作用。方法　日本纯种大耳兔 ,手术切取双肾 ,将离体肾置于 4℃的HCA灌洗液中保存 2 4h ,检测肾细胞线粒体内总钙和肾组织三磷酸腺苷 (ATP)的含量。结果　与肾脏切取后立即检测相比 ,保存 2 4h后肾细胞线粒体内钙浓度明显上升、ATP则下降 ;保存液中加入钙拮抗剂异博定 (VP)后 ,上述现象可得以显著抑制。结论　在离体肾保存过程中 ,钙拮抗剂异博定能阻止钙进入细胞线粒体内 ,该现象可能具有保护离体肾的作用。Objective To investigate the protective effect of calcium antagonist Verapamil (VP) on kidney preservation in HCA solution. Methods After kidneys were isolated from rabbits, they were perfused and stored in HCA solution or in HCA solution with VP pre supplement at 4℃ for 24 h respectively. The contents of mitochondrial calcium in renal cells and ATP in renal tissues were measured in every group. Results The contents of mitochondrial calcium was remarkably higher and ATP significantly lower in the kidneys in HCA solution at 4℃ for 24 h than those just after resection. But these could be inhibited in those storing in the HCA solution with VP pre supplement. Conclusion Calcium antagonist VP can protect kidney function during HCA solution preservation by inhibiting calcium intaking into mitochondrium.

关 键 词：离体肾脏保存 钙拮抗剂 异搏定 

分 类 号：R692[医药卫生—泌尿科学] R699.2[医药卫生—外科学]