鼠脑驱动蛋白ATP水解机制的晶体学分析  

Crystallography of ATP hydrolysis mechanism in rat brain kinesin

作　　者：万群[1] 祝娉婷[1] 吕后宁 陈欣虹[1] 

机构地区：[1]扬州大学医学院生化系,扬州江苏225001

出　　处：《生物工程学报》2014年第4期644-657,共14页Chinese Journal of Biotechnology

基　　金：扬州大学科技创新培育基金(No.2013CXJ083)资助~~

摘　　要：鼠脑驱动蛋白是一类利用ATP水解释放的能量在微管系统上高连续性运动的常规驱动蛋白。了解ATP水解的化学能如何转化为机械动能是驱动蛋白研究中的重大课题。为此,鼠脑驱动蛋白单体(rK354)的晶体通过浸泡的方式引入ATP的结构类似物AMPPNP。rK354-AMPPNP复合物和rK354-ADP复合物结构的比较,揭示了开关区域Ⅱ的Glu237起连接ATP的γ-磷酸和驱动蛋白微管结合区的枢纽作用。Rat brain kinesin is a conventional kinesin that uses the energy from ATP hydrolysis to walk along the microtubule progressively. Studying how the chemical energy in ATP is utilized for mechanical movement is important to understand this moving function. The monomeric motor domain, rK354, was crystallized. An ATP analog, AMPPNP, was soaked in the active site. Comparing the complex structure of rK354.AMPPNP and that of rK354.ADP, a hypothesis is proposed that Glu237 in the Switch II region sensors the presence of y-phosphate and transfers the signal to the microtubule binding region.

关 键 词：驱动蛋白 ATP水解 晶体结构 开关区域 

分 类 号：Q51[生物学—生物化学]