急性炎症环境下大鼠前列腺组织内腺苷和细胞因子作用的研究  

Roles of adenosine and cytokines in the prostate tissue of rats with acute bacterial prostatitis

作　　者：龙智[1] 裴夏明[1] 何乐业[1] 戴英波[1] 彭东毅[1] 张一川[1] 石玄言[1] 何景良[1] 

机构地区：[1]中南大学湘雅三医院泌尿外科,湖南长沙410013

出　　处：《中华男科学杂志》2014年第4期315-319,共5页National Journal of Andrology

基　　金：湖南省卫生厅基金(B2011-031);湖南省科技计划基金(2011FJ3129)~~

摘　　要：目的:通过研究大鼠急性细菌性前列腺炎组织中腺苷浓度以及TNF-α、IL-10的表达,探讨腺苷与炎症细胞因子在前列腺急性炎症过程中的作用。方法:建立急性细菌性前列腺炎大鼠模型,分为前列腺炎组、茶碱干预组、假手术组和空白对照组,每组12只。分别于4、14d处死半数并获取前列腺组织。免疫组化检测组织中IL-10、TNF-α的表达,高效液相色谱法检测各组组织中的腺苷浓度。结果:急性细菌性炎症环境下前列腺组织中不同处理时间的腺苷浓度[(48.38±17.27)、(26.54±11.22)μg/g]均显著高于假手术组[(0.45±0.25)、(0.46±0.29)μg/g]和空白对照组[(0.41±0.23)、(0.43±0.27)μg/g,P<0.05];TNF-α(0.23±0.08、0.21±0.03)、IL-10(0.13±0.03、0.25±0.01)的表达较假手术组(0.07±0.03、0.07±0.01;0.07±0.03、0.07±0.03)、空白对照组(0.07±0.06、0.07±0.06;0.07±0.01、0.07±0.02)显著升高(P<0.05);给予腺苷受体阻滞剂茶碱干预后,与前列腺炎组相比,干预组大鼠的前列腺组织炎症程度加重,组织中腺苷浓度显著升高[(86.64±32.87)、(51.17±22.96)μg/g,P<0.05],TNF-α的表达显著升高(0.37±0.08、0.32±0.06,P<0.05),但IL-10的表达无明显改变(0.12±0.06、0.15±0.06,P>0.05)。结论:腺苷可能通过腺苷受体信号通路调节大鼠急性细菌性前列腺炎组织中细胞因子IL-10、TNF-α的表达,影响组织炎症的进程。Objective ： To investigate the possible roles of adenosine and the cytokines TNF-α and IL-10 in the pathogenesis of acute bacterial prostatitis （ABP） in rats. Methods： Forty-eight male Wistar rats were randomly divided into groups A （ABP）, B （ABP ＋ theophylline intervention）, C （sham） and D （blank control）. ABP models were established by injecting Escherichia coli O157 into the prostate, and those in group B were treated by intraperitoneal injection of theophyUine immediately after modeling. At 4 and 14 days, the prostate tissues of the rats were collected for detection of the expressions of TNF-α and IL-10 by immunohistochemistry and the concentration of adenosine by high-performance liquid chromatography. Results： At 4 and 14 days, the concentrations of adenosine were significantly higher in group A （ [ 48.38 ± 17.27 ] and [ 26.54 ± 11.22 ] μg/g） than in C （ [ 0.45 ± 0.25 ] and [0. 46 ± 0. 29 ] μg/g） and D （ [ 0.41 ± 0.23 ] and [ 0.43 ± 0.27 ] μg/g） （P 〈 0. 05 ）, and so were the expressions of TNF-α in A （0.23 ± 0.08 and 0.21 ±0.03） than in C （0.07 ±0.03 and 0.07 ±0.01 ） and D （0.07 ±0.06 and 0.07 ±0.06） （P 〈0.05）, and those of IL-IO in A （0.13 ±0.03 and 0.25 ±0.01） than in C （0.07 ±0.03 and 0.07 ±0.03） and D （0.07 ±0.01 and 0.07 ±0.02） （P〈 0. 05 ）. Compared with group A, the rats in group B showed significant increases at 4 and 14 days in the severity of inflammation, concentration of adenosine （ [ 86.64 ± 32.87 ] and [ 51.17 ± 22.96] μg/g, P 〈 0. 05 ） and expression of TNF-α （ 0.37 ± 0. 08 and 0. 32 ± 0. 06, P 〈 0.05 ）, but exhibited no remarkable difference in the expression of IL-10 （0.12 ± 0.06 and 0.15 ± 0. 06, P 〉 0.05 ）. Conclusion ： Adenosine may affect the progression of inflammation by regulating the expressions of the cytokines TNF-α and IL-10 in ABP rats through the adenosine receptor signaling pathway. Natl J Adrol, 2014, 20 （4） ： 315 -319

关 键 词：急性细菌性前列腺炎 腺苷信号通路 细胞因子 大鼠 

分 类 号：R697.33[医药卫生—泌尿科学]