不同脂肪含量的肌肉食物对丝光绿蝇生长发育的影响  

Effect of Diets with Different Fat Levels on the Body Size and Development of Lucilia sericata

作　　者：李学博[1] 郑士立[2] 王清山[3] 丁春丽[1] 李红卫[3] 杨永强[4] 万立华[4] 

机构地区：[1]山东省高校证据鉴识重点实验室,山东政法学院,济南400063 [2]西南政法大学法学院,重庆401120 [3]重庆市公安局物证鉴定中心,重庆400021 [4]重庆医科大学法医学教研室,重庆400016

出　　处：《中国寄生虫学与寄生虫病杂志》2014年第2期135-138,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：公安部重点研究计划项目(No.2011ZDYJCQSJ006);重庆市公安局科技攻关计划项目(No.2012-05)~~

摘　　要：目的观察不同脂肪含量的肌肉食物对丝光绿蝇生长发育的影响。方法在28℃恒温条件下,分别利用含0%(G0组)、10%(G1组)、30%(G3组)、50%(G5组)和80%(G8组)脂肪质量比的肌肉混合培养基饲养丝光绿蝇初孵幼虫。自幼虫孵化后16 h起,每12 h测量各组幼虫体长和体重,蛹长和蛹重,以及成虫左翅中横脉长度,每次取样10只。计算各组发育历期,统计幼虫及蛹的死亡率和成虫的性别比率。结果 G1、G3、G5和G8组幼虫的平均最大体长[(13.3±1.2)、(12.0±1.1)、(10.2±0.9)和(8.8±0.8)mm]和平均最大体重[(72.8±6.1)、(62.2±5.7)、(47.2±4.3)和(34.9±5.7)mg]均显著小于G0组[分别为(14.8±1.3)mm和(80.4±8.1)mg](P<0.01),蛹和成虫的个体大小亦显著小于G0组(P<0.01)。G5和G8组幼虫的总发育历期分别为(293.3±22.2)h和(285.2±24.6)h,显著短于G0组[(312.8±20.1)h](P<0.01),其幼虫死亡率[(32.6±5.6)%和(44.3±7.7)%]和蛹死亡率[(28.6±5.5)%和(43.5±6.2)%]则显著高于G0组幼虫死亡率[(5.7±3.3)%]和蛹死亡率[(4.5±1.9)%](P<0.01)。各组成虫的性别比率间差异无统计学意义(P>0.05)。结论肌肉食物内脂肪含量增高可使丝光绿蝇幼虫、蛹和成虫个体明显变小,且发育历期缩短,死亡率增高。Objective To observe the effect of diets with different fat levels on the body size and development of Lucilia sericata. Methods Under the constant temperature of 28 ~C, the larvae were reared on the diets containing 0%（G0）, 10%（G1）, 30%（G3）, 50%（G5） and 80%（G8） fat tissues （fat/muscle ratio）, respectively. Length and weight of larvae and pupae were measured at 12 h interval since 16 h after eclosion. Length of inter-medial cross vein（m-m） of adult left wing was measured. 10 samples were collected in each group. The developmental duration time, mortality and sex ratios of adults were recorded. Results The mean maximal larval length [（13.3±1.2）, （12.0±1.1）, （10.2± 0.9） and （8.8±0.8） mm, respectivelyl and mean maximal larval weight [（72.8±6.1）, （62.2±5.7）, （47.2±4.3）, and （34.9±5.7） mg] in G1, G3, G5 and G8 groups were significantly less than that of the GO group [（14.8±1.3） mm and （80.4±.1） mg^（P〈0.01）. The body size of pupae and adults was also significantly less than that of GO group （P〈 0.01）. The total duration time of G5 and G8 groups [（293.3±2.2） and （285.2±4.6） hi were significantly shorter than that of GO group [（312.8±0.1）hi（P〈0.01）. The mortality of larvae [（32.6±.6）% and （44.3±.7）%] and pu- pae[（28.6±.5）% and （43.5±.2）%1 of G5 and G8 group were also significantly higher than that of GO group[（5.7±3.3）% and （4.5±.9）%]（P〈0.01）. There was no significant difference in sex ratio among the 5 groups （P〉0.05）. Conclusion The body size of larvae, pupae and adults of Lucilia sericata is smaller, the development time is shorter and mortality is higher when the food substrate contains more fat tissues.

关 键 词：法医昆虫学 丝光绿蝇 发育历期 食物来源 脂肪 

分 类 号：D919.1[医药卫生—法医学]