白细胞介素37对THP-1源巨噬细胞泡沫化的影响  

Effect of IL-37 on THP-1 differentiated foam cells formation

作　　者：黄婷[1] 王博远[2] 任泽鹏 王祥[2] 李大主[2] 

机构地区：[1]武汉市第一医院老年病科,武汉430022 [2]华中科技大学附属协和医院心内科 [3]鄂尔多斯市中心医院心内科

出　　处：《临床心血管病杂志》2014年第4期338-341,共4页Journal of Clinical Cardiology

基　　金：国家自然科学基金资助项目(No:81370406)

摘　　要：目的:研究白细胞介素(白介素)-37对人THP-1源巨噬细胞泡沫化的影响及机制。方法:体外培养THP-1,用佛波酯(PMA)刺激24h使其分化为巨噬细胞后,进行以下处理:①用IL-37和(或)ox-LDL刺激THP-1源巨噬细胞24h;②用不同浓度的IL-37和ox-LDL同时刺激24h;③用ox-LDL刺激THP-1源巨噬细胞24h,再加IL-37干预24h,收集细胞。采用油红染色观察各组泡沫细胞所占细胞总数的比例,TC试剂盒测定细胞内TC的含量,利用RT-PCR、Western blot实验方法从mRNA水平和蛋白水平测定泡沫细胞清道夫受体CD36和SRA和ABCA-1的表达。结果:与对照组比较,IL-37能够明显减少泡沫细胞的形成,降低泡沫细胞TC的聚集[(303.10±8.90)ng/mg∶(150.40±7.36)ng/mg,P<0.01],显著抑制巨噬细胞清道夫受体CD36和SRA的表达,并增强ABCA-1的表达,且作用效果与溶度呈正相关。对ox-LDL诱导的泡沫细胞,给予IL-37后仍能够显著上调ABCA1的表达,显著下调SRA、CD36的表达。结论:白细胞介素-37可抑制THP-1源巨噬细胞的泡沫化,其作用机制可能是通过IL-37抑制清道夫受体CD36和SRA、促进ABCA-1的表达实现的。Objective：To investigate how IL-37 affects macrophages foam-cell formation, and thereby investi- gate the mechanism of IL-37 in the development of atherosclerosis. Method： THP-1 cells were differentiated into macrophages using 160 nMPMA for 24 h. And then with ox-LDL or IL-37 combine with ox-LDL, thereof in growth medium for 24 hours to transform macrophages into foam cells. THP-1 cells differentiated macrophages were stimulated with ox-LDL for 24 h, then with 10 ng/ml IL-37 for another 24 hours. Oil red O staining and cel- lular lipid measurement were used to identify macrophage foam cell formation. Real-time PCR and Western blot were carried out to explore the mechanism of IL-37-mediated suppression on THP-l-macrophages derived foam cell formation. Result：Oil Red O staining identified foam cell formation was significantly reduced in cells treated with IL-37 and ox-LDL. The similar effect of IL-37 was obtained when extracted oil red O and measured by a spectro- photometer. Compared with ox-LDL alone, IL-37 and ox-LDL significantly reduced the lipids accumulation in macrophage foam cells in a concentration-dependent manner. Total cellular cholesterol was significantly reduced in IL-37 and ox-LDL cultures relative to ox-LDL alone [（150.4±7.36）ng/mg vs （303.1±8.9）ng/mg, P〈0.01]. Moreover, real-time PCR and Western blot analysis showed that the expression of both CD36 and SRA of macro- phage foam cells treated by ox-LDL together with IL-37 was significantly down-regulated, while ABCA-1 up-regu- lated significantly. Conclusion.. IL-37 may inhibit THP-1 differentiated macrophage foam-cell formation, which is largely caused by a down-regulated expression of scavenger receptor SRA and CD36 and up-regulated expression of ABCA-1.

关 键 词：IL-37 清道夫受体 THP-1 泡沫细胞 

分 类 号：R541.4[医药卫生—心血管疾病]