去细胞组织工程猪肺动脉带瓣管道的生物相容性研究  

The biological properties of decellularized porcine tissue-engineering pulmonary valved conduits

作　　者：王圣[1] 李温斌[2] 武喜红[3] 程兆云[1] 赵子牛[1] 

机构地区：[1]河南省人民医院心外科 [2]首都医科大学附属北京安贞医院 [3]郑州市第一人民医院

出　　处：《临床心血管病杂志》2014年第4期346-348,共3页Journal of Clinical Cardiology

摘　　要：目的:探讨去细胞组织工程猪肺动脉带瓣管道的生物相容性。方法:①新鲜猪肺动脉带瓣管道组织3份,A组为对照组,B、C组采用胰蛋白酶+Triton X-100对新鲜猪肺动脉带瓣管道进行去细胞处理,C组在去细胞处理后行明胶嵌合再处理;②对3组瓣膜及管壁组织行大鼠皮下包埋实验,8周后观察材料的组织相容性和生长情况;③通过动态凝血试验、溶血试验、血小板粘附性能试验分析3种组织材料的血液相容性。结果:与A组相比,B组及C组的炎性细胞浸润显著减少,C组仅有轻微组织反应;B、C组材料组织相容性好,并于8周后有纤维细胞长入。相对A组及B组,C组的瓣膜及管壁组织具有更低的凝血率,OD-凝血时间曲线下降更缓慢且吸光度高,同时血小板粘附更少且没有明显变形和伪足。结论:这种去细胞组织工程猪肺动脉带瓣管道不仅具有极低的免疫原性,而且具有一定的生长潜能、良好的组织相容性及血液相容性,有可能成为一种新的、理想的组织工程肺动脉带瓣管道支架材料。Objective：To explore the biological properties of decellularized porcine tissue-engineering pulmona- ry valved conduits. Method.. The fresh porcine pulmonary valved conduits were divided into three groups. Group A was control group. Group B and C were decellularized with trypsin~ Triton X-100. Group C was retreated by gelatin mosaicing. Three sets of valves and wall tissue were implanted subcutaneously in mice and extirpated at 8th week in order to study its biocompatibility and biodegradibility. Three kinds of material hemocompatibility were analyzed through the dynamic blood clotting test, hemolysis test, platelet adhesion performance test. Result：Com- pared to the A group, the infiltration of inflammatory cells significantly reduced in B group and C group, the valve and wall in C group showed extremely light tissue reaction. The latter two groups had good histocompatibility and growth function, and the decellular valve and wall were completely absorbed without fibrosis or scar formation in the implantation area by the end of the 8th week. Compared with A group and B group, valves and wall in C group had a lower coagulation rate, OD-clotting time curves declined more slowly and the absorbance value was high. At the same time, platelet adhesions were fewer, and had no apparent deformation and pseudopodium. Conclusion： The new gelatin mosaic acellular porcine pulmonary valved conduits not only can further reduce heterogeneic pul- monic immunogenicity, but also have good growth potential and histocompatibility, which has the potential to become a new and ideal tissue engineering pulmonary valved conduits scaffold.

关 键 词：去细胞 肺动脉带瓣管道 组织工程 

分 类 号：R33[医药卫生—人体生理学]