月经周期中不同类型人子宫内膜体外培养的细胞形态学特点  

Morphological Characteristics of Different Endometrial Pattern Cells Cultured in vitro

作　　者：刘明慧[1] 晁贺[1] 赵琰誉[1] 高秀霞[1] 王欢[1] 刘英[1] 

机构地区：[1]首都医科大学附属北京妇产医院生殖中心,北京100026

出　　处：《生殖与避孕》2014年第4期263-268,共6页Reproduction and Contraception

基　　金：北京市自然科学基金项目(5122015);北京市科技新星基金项目(H020821200190);首都医科大学附属北京妇产医院院内基金项目(201114)

摘　　要：目的:探讨不同类型子宫内膜细胞体外培养的细胞形态学特点。方法:在月经周期的不同时期,通过B超检测判断患者子宫内膜的厚度和类型,取不同形态分型的人子宫内膜进行体外培养,倒置显微镜下观察子宫内膜体外培养的细胞形态学特性,并通过免疫荧光法进行鉴定,采用培养板单层贴壁细胞的原位计数方法,计算子宫内膜间质细胞和腺上皮细胞数量及比例。结果:不同类型子宫内膜经培养后,其中间质细胞形态呈扁平状,胞质透明,核圆、居中,波形蛋白表达阳性;腺上皮细胞呈多角形或蝌蚪形,旋涡状排列,核圆而大,角蛋白表达阳性。A型子宫内膜组织腺细胞贴壁率为85.8±5.7%,贴壁培养后腺上皮细胞的比例占43.3±11.7%,间质细胞比例占56.7±11.7%;B型子宫内膜组织腺细胞贴壁率为8.7±3.9%,贴壁培养后腺上皮细胞的比例占13.3±3.3%,间质细胞比例占86.7±3.3%;C型子宫内膜组织腺细胞贴壁率为6.0±1.9%,贴壁培养后腺上皮细胞的比例占12.3±3.0%,间质细胞比例占87.7±3.0%。A组与B组、C组两组腺上皮细胞贴壁率及贴壁后腺细胞比例有明显统计学差异(P<0.05),B、C组之间腺上皮细胞贴壁率及腺细胞比例无明显统计学差异(P>0.05)。结论:B、C型子宫内膜体外培养腺上皮细胞贴壁比例低于间质细胞,体外培养后以间质细胞为主;A型子宫内膜细胞培养所得的间质细胞及腺细胞均较多,腺细胞贴壁率及腺细胞比例明显高于B、C型子宫内膜,是用于胚胎着床机制等实验研究较好的标本来源。Objective： To investigate the morphological characteristics of different human endometrial patterns by cell culture in vitro. Methods： Using B-mode ultrasound, the thinkness and patterns of endometrium were observed, and then different patterns of endometrium were cultured in vitro. The endometrial cells were then observed by a light microscopy and verified by immunofluorescence. By counting cell numbers under microscopy randomly, the endometfial stromal cells and glandular epithelial cell proportion were calculated. Results： Endometfial stromal cells were fiat, transparent cytoplasm, nuclear circle center and positive for vimentin monoclonal antibody. Glandular epithelial cells were polygonal or tadpole shape, spiral arrangement, nuclear big and positive for keratin monoclonal antibody staining. The adherent rate of Type A endometrial glandular cell was 85.8 ± 5.7%, and the proportion of glandular epithelial cells was 43.3±11.7%, stromal cells proportion was 56.7±11.7%. The adherent rate of type B endometfial glandular cell was 8.7 ± 3.9%, and the proportion of glandular epithelial cells was 13.3 ± 3.3%, the proportion of stromal cells was 86.7 ± 3.3%. The adherent rate of type C endometrial glandular cell was 6.0 ±1.9%, and the proportion of glandular epithelial cells was 12.3 ±3.0%, the proportion of stromal cells was 87.7± 3.0%. The adherent rate and glandular cell percentage were significantly different between type A and type B or type C （P〈0.05）, and there were no significantly differences between type B and type C （P〉0.05）. Conclusion： The adherence rate of glandular cells was less than stromal cells in type B and type C. There were mainly stromal cells alive after culture. However, both the stromal cells and glandular cells were affluent after culture in type A, which means a better choice for the research of embryo implantation.

关 键 词：子宫内膜 细胞培养 子宫内膜形态分型 

分 类 号：R711[医药卫生—妇产科学]