右美托咪定对缺血-再灌注大鼠肠黏膜屏障功能的影响  

Effects of dexmedetomidine on function of intestinal mucosal barrier of rats with ischemia-reperfusion injury

作　　者：张瑛[1] 钟文晖[1] 王爱忠[1] 

机构地区：[1]上海交通大学附属第六人民医院麻醉科,上海201306

出　　处：《上海交通大学学报（医学版）》2014年第4期487-490,506,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的观察右美托咪定对肠缺血-再灌注损伤(IRI)大鼠肠型脂肪酸结合蛋白(I-FABP)及炎症因子的影响,并探讨其作用机制。方法建立大鼠肠IRI模型,随机分为假手术组、模型组、右美托咪定组。分别于缺血-再灌注后2 h和6 h时间点抽取大鼠腹主动脉血,采用酶联免疫吸附试验(ELISA)法检测大鼠血清I-FABP水平;于实验终点留取肠组织,ELISA法检测白介素-6(IL-6)和IL-10的含量,苏木精-伊红(HE)染色后经Chiu六级评分法判定大鼠小肠组织损伤程度。结果与假手术组比较,模型组和右美托咪定组再灌注2 h和6 h时血清I-FABP水平明显升高,差异有统计学意义(P<0.05);与模型组比较,右美托咪定组血清I-FABP含量显著下降,差异有统计学意义(P<0.05)。模型组和右美托咪定组肠组织中IL-6和IL-10的含量均明显高于假手术组,差异有统计学意义(P<0.05);与模型组比较,右美托咪定组肠组织中IL-6含量降低,IL-10含量升高,差异均有统计学意义(P<0.05)。右美托咪定组Chiu评分显著低于模型组,但高于假手术组,差异均有统计学意义(P<0.05)。结论右美托咪定可有效减少肠缺血-再灌注后炎症因子的产生,降低血浆中I-FABP水平,具有肠道保护作用。Objective To observe the effects and to discuss the mechanism of dexmedetomidine on the intestinal fatty acid binding protein （I-FABP） and inflammatory factors of rats with intestinal ischemia-reperfusion injury. Methods The intestinal ischemia reperfusion injury model of rats was established and then rats were randomly divided into the sham operation group, ischemia-reperfusion group, and dexmedetomidine group. The aortic blood of rats was extracted at the 2 h and 6 h after ischemia-reperfusion. The plasma I-FABP levels were measured by ELISA. By the end of experiments, the contents of IL-6 and IL-10 in intestinal tissues were measured by ELISA. The intestinal tissues were stained by the HE and their injury severity was scored by the methods of Chin＇s six levels. Results The plasma I-FABP levels of the ischemia-reperfusion group and dexmedetomidine group at 2 h and 6 h after reperfusion were significantly higher than those of the sham operation group. The differences were statistically significant （P〈0.05）. Compared to the ischemia-reperfusion group, the plasma I-FABP levels of the dexmedetomidine group were significantly decreased. The differences were statistically significant （P〈0.05）. The contents of IL-6 and IL-10 in intestinal tissues of the ischemia- reperfusion group and dexmedetomidine group were significantly higher than the sham operation group. The differences were statistically significant （P〈0.05）. Compared to the ischemia-reperfusion group, the contents of IL-6 in intestinal tissues of the dexmedetomidine group were lower, while the contents of IL-10 were higher. The differences were statisdcaUy significant （19 〈 0. 05）. The Chiu＇s score of the dexmedetomidine group was significandy lower than that of the ischemia-reperfusion group but higher than that of the sham operation group.The differences were statistically significant （P 〈0.05）. the production of inflammatory factor after intestinal I-FABP, and protect the intestinal functions. Conclusion Dexmedetomid

关 键 词：右美托咪定 缺血-再灌注 炎症因子 肠型脂肪酸结合蛋白 

分 类 号：R614[医药卫生—麻醉学]