重症中暑大鼠肠系膜淋巴微循环变化  

Changes in mesenteric lymph microcirculation in rats suffering from severe heatstroke

作　　者：童华生[1] 段鹏凯[1] 张兴钦[1] 万鹏[1] 唐柚青[1] 唐丽群[1] 苏磊[1] 

机构地区：[1]广州军区广州总医院重症医学科、全军热区创伤救治与组织修复重点实验室,广州510010

出　　处：《解放军医学杂志》2014年第4期329-332,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(81101406);军队“十二五”医学科研基金重点项目(BWS12J108)~~

摘　　要：目的观察重症中暑肠系膜淋巴微循环和动力学变化规律,探讨肠系膜淋巴在重症中暑发病机制中的作用。方法制备重症中暑大鼠模型,观察热应激时大鼠生命体征的变化以及重症中暑的发生时间。分别于热暴露前、热暴露后60min、重症中暑发生时动态观察大鼠肠系膜淋巴微循环参数Index-Ⅰ、Index-Ⅱ和L.D-Index及淋巴管压力变化,每间隔30min动态计量肠系膜淋巴生成量。结果成功建立了稳定的重症中暑大鼠模型,大鼠中心体温达42℃的时间约为60min,而重症中暑发生时间约为77min。大鼠于热环境下暴露60min,肠系膜淋巴管自主收缩频率、Index-Ⅰ、Index-Ⅱ、L.D-Index、淋巴管压力和淋巴生成量明显下降(P<0.05),而在重症中暑发生时肠系膜淋巴管自主收缩频率、收缩活性指数Index-Ⅰ、Index-Ⅱ、D-Index、淋巴管压力和淋巴生成量较中心体温为42℃时有所上升(P<0.05),但未达到热暴露前水平(P<0.05)。结论重症中暑大鼠的肠系膜淋巴微循环变化具有一定动态规律,可能参与了重症中暑的发病机制。Objective To observe the dynamic changes in mesenteric lymph microcirculation and its kinetics in rats suffering from severe heatstroke （SHS）, and to explore the role ofmesenteric lymph in the pathogenesis of SHS. Methods SHS rat models were reproduced in an incubator with high temperature and high humidity. The vital signs and the time of onset of SHS in rats were recorded continuously during the process of heat stress. Parameters of mesenteric lymph microcirculation including Index-Ⅰ, Index-Ⅱ, L.D-Index, and intra-lymphatic pressure before heat exposure, 60min after heat exposure, and onset of SHS were collected and analyzed. Mesenteric lymph was collected at 30-min interval, and its volume of production was measured dynamically. Results Rat SHS model was reproduced successfully. After exposure to the environment with high temperature and high humidity, the core temperatures of the rats raised to 42 ℃ at the time point of 60min, and HS onset occurred at about 77min. Mesenteric lymph-vessel contraction indices including Index-Ⅰ, Index-Ⅱ, L.D-Index, lymph-vessel pressure and mesenteric lymph flow decreased significantly at the time point of 60min （P〈0.0S）. However, all the above parameters increased at the time point of SHS onset （P〈0.05）, but had not yet reached the normal levels before hyperthermia and high humidity exposure （P〈0.05）. Conclusion Changes of mesenteric lymph microcirculation in rats with SHS shows a dynamical regularity, which may take part in the pathogenesis of SHS.

关 键 词：中暑 热应激障碍 肠系膜 淋巴 微循环 

分 类 号：R594.12[医药卫生—内科学]