α-生育酚琥珀酸酯对急性放射病小鼠早期造血损伤的防护作用观察  

Protective effect of α-tocopherol succinate against early hematopoietic injury in mice with acute radiation sickness

作　　者：贾超[1,2] 张刘珍[2] 邢爽[2] 柳晓兰[2] 善亚君[2] 丛悦[2] 崔宇[2] 王丽梅[2] 从玉文[2] 

机构地区：[1]广西医科大学研究生院,南宁530000 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《解放军医学杂志》2014年第4期333-337,共5页Medical Journal of Chinese People's Liberation Army

摘　　要：目的探讨α-生育酚琥珀酸酯（α-TOS）对急性放射病小鼠早期造血损伤的防护作用。方法选取6～8周龄C57BL／6j雄性小鼠，随机分为正常对照组、照射对照组和α-TOS组（n=10）。α-TOS组照射前24h皮下注射400mg／kgα-TOS，照射对照组给予等量辅剂。观察9．0Gy全身照射小鼠的30d存活率及死亡动物平均生存时间。定期检N6．5Gy全身照射小鼠外周血象。计数6．5Gy照射后2、24h骨髓细胞集落和干／祖细胞数。将6．5Gy外射小鼠骨髓细胞与EGFP转基因小鼠骨髓细胞按10：1比例混合后移植入致死剂量照射小鼠体内，检测移植后35d受体小鼠外周血中EGFP阳性细胞比例。结果d-TOS可明显提高9．0Gy照射小鼠的30d存活率，延长死亡动物平均生存时间，促进6．5Gy照射小鼠外周血象的恢复，提高照射后24h／b鼠骨髓细胞集落生成能力和干／祖细胞数目。竞争移植实验中，仅．TOS可明显降低致死剂量照射小鼠移植后3sd外周血中EGFP阳性细胞比例。结论d．TOS可有效保护γ射线照射小鼠照后早期的造血干／祖细胞，促进外周血象恢复，增加存活率。Objective To explore the protective effect of α -tocopherol succinate （ α -TOS） against early hematopoietic injury in mice with acute radiation sickness. Methods Male C57BL/6J mice 6-8 weeks old were randomly divided into normal group, irradiation group and α -TOS group （n=10）, 400mg/kg of α -TOS was subcutaneous injected into the mice of ot -TOS group 24 hours before irradiation, while vehicle was given to the mice of irradiation group. The 30-day survival rate of mice having received 9.0Gy irradiation, and the mean survival time of non-survivors were recorded. Analysis of peripheral blood was performed in mice receiving 6.SGy irradiation at days 1, 4, 7, 10, 14, 18, 22, 31 and 45, while the number of bone marrow colony-forming cells （CFCs） and hernatopoietic stem/progenitor cells were counted at 2-hour and 24-hour after irradiation. Bone marrow cells collected from 6.5Gy irradiated mice and EGFP transgenic mice were mixed in a ratio of 10：1, and then transplanted into lethal dose-irradiated mice, and the proportion of EGFP positive cells in the peripheral blood was recorded after 35 days. Results α -TOS raised the survival rate and mean survival time of mice suffering from 9.0Gy irradiation, improved the recovery of the peripheral blood picture of mice suffering from 6.SGy irradiation, and increased the number of bone marrow colony-forming cells and hematopoietic stem/progenitor cells in the mice 24 hours after 6.SGy irradiation. The competitive transplantation experiments showed that tx -TOS markedly diminished the proportion of EGFP positive cells in the peripheral blood in lethal dose-irradiated mice at day 35. Conclusion ct -TOS could effectively protect the hematopoietic stem/progenitor cells from radiation injury in γ -ray irradiated mice, promote the recovery of peripheral blood cells, and improve the survival rate.

关 键 词：α-生育酚琥珀酸酯 辐射损伤 实验性 造血干细胞 竞争移植实验 

分 类 号：R818.05[医药卫生—放射医学]