stim1和orai1蛋白在哮喘大鼠支气管平滑肌中的表达  

The expression of stim1 and orai1 protein in asthmatic rats airway smooth muscle cells

作　　者：王丽[1] 丁圣刚[1] 李忠强[2] 王亚亭[1] 

机构地区：[1]安徽医科大学第一附属医院儿科,合肥230022 [2]临沂市人民医院儿科,临沂276000

出　　处：《安徽医科大学学报》2014年第4期430-433,共4页Acta Universitatis Medicinalis Anhui

摘　　要：目的研究基质交感分子1(stim1)和钙离子释放激活钙离子通道蛋白1(orai1)在哮喘气道平滑肌细胞中表达水平的变化。方法选取40只清洁级SD大鼠随机分为对照组和哮喘组,其中哮喘组用鸡卵清蛋白(OVA)激发制备大鼠哮喘模型(n=20),对照组用生理盐水代替(n=20)。肺组织病理切片HE染色判断哮喘模型是否建立成功,分析支气管肺泡灌洗液(BALF)中的细胞总数及分类计数,应用免疫组化技术及彩色病理图文分析系统测定stim1和orai1蛋白阳性细胞平均光密度值。结果肺组织病理切片HE染色镜下观察呈现大量炎细胞浸润,支气管黏膜肿胀,支气管腔内可见黏液栓等,证实哮喘模型建立成功。哮喘组中大鼠BALF中细胞总数、中性粒细胞、嗜酸性粒细胞计数明显高于对照组,差异有统计学意义(P<0.01)。哮喘组大鼠支气管平滑肌表达orai1蛋白阳性细胞平均光密度值明显高于对照组(P<0.05)。哮喘组和对照组大鼠支气管平滑肌表达stim1蛋白阳性细胞的平均光密度值无明显差异(P>0.05)。结论 orai1蛋白在哮喘大鼠气道平滑肌细胞中表达变化较stim1蛋白明显,对支气管平滑肌调节作用可能更重要。Objective To investigate the changes of stiml and orail protein in the level of expression in asthmatic airway smooth muscle cells. Methods 40 SD rats were randomly divided into normal control group and asthma group, and asthma model was established by OVA sensitization in rats. HE staining of lung tissue to determine whether the asthma model successfully established. Cell counting and classification were obtained in bronchoalveo- lar lavage fluid （BALF） of rats, immunohistochemical detection and color graphic analysis system was used to measure the expression of stiml and orail protein positive cells mean optical density value. Results The lung tis- sue pathological section HE staining showed inflammatory cell infiltration, bronchial mucosal edema, bronchial visi- ble mucus, confirmed the asthma model was established successfully. Compared with the normal control group, the total number of cells, neutrophils count, eosinophils count in BALF sediment of the asthma group were significantly increased, and the difference was statistically significant （P 〈 0. 01 ）. The expression of orail protein positive cells mean optical density value of asthma group was significantly higher than the normal control group （P 〈 0.05 ）. Asthma group and normal control group had no significant difference in expression the stiml protein positive cells in the average optical density value（P 〉0. 05）. Conclusion Orail proteins may play an important role in the con- traction of asthma rat airway smooth muscle cells.

关 键 词：基质交感分子1 orai1蛋白 哮喘 平滑肌细胞 

分 类 号：R562.25[医药卫生—呼吸系统]