柔嫩艾美耳球虫对氯嗪苯乙氰和常山酮抗性快速诱导(英文)  

Rapid Development of Drug-resistant Strains of Eimeria tenella to Diclazuril and Halofugenone in Laboratory

作　　者：李安兴[1] 谢明权[1] 蔡建平 吴惠贤 廖翔华[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）》2001年第1期77-80,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金资助项目(994118)

摘　　要：诱导了柔嫩艾美耳球虫（Ｅｉｍｅｒｉａ ｔｅｎｅｌｌａ）甘肃株对两种药物－氯嗪苯乙氰和常山酮的抗药性.增加实验动物数和球虫的接种剂量可加快柔嫩艾美耳球虫对氯嗪苯乙氰和常山酮抗药性的产生．每组动物用１００只２～３周龄ＡＡ肉鸡，每只鸡接种２×１０６个孢子化卵囊，分别从氯嗪苯乙氰的质量比为０．１２５×１０－６和常山酮的质量比为０．６×１０－６的起始浓度开始，逐渐增加药物浓度，结果表明诱导柔嫩艾美耳球虫对ｗ（氯嗪苯乙氰）１．５×１０－６产生抗药性仅需传５代，对ｗ（常山酮）５.０×１０－６产生抗药性需６代．传代株在没有药物的情况下传５代后仍不能恢复对氯嗪苯乙氰和常山酮的敏感性，这一结果表明人工诱导对氯嗪苯乙氰和常山酮的抗药性是稳定的．实验检测了７种药物：盐霉素、莫能霉素、马度拉霉素、拉萨洛菌素、球痢灵、尼卡巴嗪和克球粉对传代株的敏感性，结果显示这７种药物和氯嗪苯乙氰及常山酮无交叉抗药性．The development of resistance of the CS strain of Eimeria tenella to the anticoccidial drugs halofugenone and diclazuril has been studied. Resistance to halofugenone and diclazuril developed readily in experiments where a large number of coccidia were exposed to the drugs, either by increasing the number of oocysts in the inoculum or by increasing the num-ber of birds in the group. When 100 birds were given 2 × 106 oocysts commenced from an initial concentration of 0.6 × 10-6 halofugenone or 0. 125 × 10-6 diclazuril, resistance to halofugenone and diclazuril appeared after 6 and 5 passages respectively. The passaged lines did not recover their sensitivity to these 2 chemicals after 5 further passages in absence of the use of drug, suggesting that the resistance developed was stable. 7 anticoccidial drugs, salinomycin, monensin, maduramycin, lasalocid, zoalene, nicarbazin and clopidol were effective against halofugenone- and diclazuril-resistant line of E. tenella, suggesting that halofugenone an d diclaruril did not exhibit cross-resistance with the above-mentioned compounds.

关 键 词：柔嫩艾美耳球虫 抗药性 氯嗪苯乙氰 常山酮 快速诱导 鸡 接种剂量 

分 类 号：S858.31[农业科学—临床兽医学] S852.723[农业科学—兽医学]