肥胖大鼠模型脑组织中GIRK4的表达研究  

Expression of GIRK4 in Brain Tissue in Diet- Induced Obesity Rats

作　　者：杨海[1] 胡燕荣[2] 李南方[2] 张向阳[1] 高莉[2] 周玲[2] 洪静[2] 

机构地区：[1]新疆医科大学,乌鲁木齐830001 [2]新疆维吾尔自治区人民医院高血压中心,新疆高血压研究所

出　　处：《医学研究杂志》2014年第4期60-63,共4页Journal of Medical Research

基　　金：国家自然科学基金地区科学项目(81160109)

摘　　要：目的研究饮食诱导肥胖大鼠G-蛋白偶联(门控)内向整流钾离子通道(GIRK4)基因在脑组织中的表达,探讨GIRK4与肥胖的相关性。方法 30只SD大鼠,雌雄各半,对照组(n=10)予以普通饲料饲喂作为,实验组(n=20)以高脂肪高热量饮食饲喂8周,建立肥胖大鼠模型,肥胖大鼠体重超过正常饮食饲喂大鼠20%以上为成功模型,处死大鼠,提取两组大鼠的脑组织,提取脑组织中的总蛋白,应用Western blot法检测GIRK4蛋白表达,Quantity One软件处理实验结果。结果 (1)15只大鼠进入肥胖组(体重大于正常对照组20%)。(2)肥胖组血清总胆固醇及甘油三酯水平高于对照组(P<0.05),高脂高糖饮饲喂建立大鼠模型,肥胖大鼠脑中表达GIRK4蛋白较正常饲喂大鼠低,两组间差异有统计学意义(P<0.05)。结论 GIRK4在肥胖大鼠脑组织中表达水平降低,且在肥胖大鼠中表达,可能与饮食诱导肥胖的发生相关。Objective To investigate GIRK4（G protein-coupled inward rectifier K ＋ channels 4 gene） expression in brain tissue of diet induced obesity rat medel,and speculate the correlation between GIRK4 and obesity.Methods Thirty SD rats were randomly assigned to obesity group（n =20） as fed by high-fat and high-sugar diet for establishment of obesity model and normal control group（n =10） as fed by normal diet.The rats were sacrificed followed by separation of brain tissue.Protein of brain tissue was extracted for detection of GIRK4 expression via Western-blotting.The weight,length and fat coefficient were compared.Results Obese rats yielded markedly higher weight（381.78 ± 73.58 vs 285.57 ± 59.17）,length （24.87 ± 2.23 vs 22.40 ± 2.01）,fat coefficient （3.17 ± 1.06 vs 1.10 ± 0.43）,（P ＜ 0.05,respectively）,yet significantly suppressed GIRK4 expression in brain tissue （P ＜ 0.05）.Conclusion The down-regulation of GIRK4 expression in brain tissue may be related to fat metabolism and obesity induced by diet.

关 键 词：肥胖大鼠 GIRK4 脑组织 

分 类 号：R393[医药卫生—基础医学]