白血病抑制因子在小鼠胚胎着床机制的体外探究  

In Vitro Study of Leukemia Inhibitory Factor in Mouse Embryo Implantation

作　　者：叶天民[1] 肖佳[2] 杨健之[1] 范宇平[1] 滕晓明[1] 

机构地区：[1]同济大学附属第一妇婴保健院生殖医学中心,上海200040 [2]暨南大学免疫生物学系,广州510632

出　　处：《医学研究杂志》2014年第4期111-115,共5页Journal of Medical Research

摘　　要：目的运用已有的小鼠体外三维着床模型观察LIF抑制状态下对胚胎着床这一过程中的影响以及其转导通路中下游STAT3和pSTAT3蛋白表达水平的变化,从而探究LIF及其转导通路在胚胎着床中的功能。方法将小鼠内膜组织置于有不同浓度LIF抗体(0.1和1.0μg/ml)及正常IgG对照和阴性对照的培养基中预培养0.5h。然后进行内膜组织和囊胚的共培养,观察比较胚胎与内膜的黏着率的不同,并对抗体处理后的内膜进行STAT3及pSTAT3的免疫组化染色。结果运用1.0%抗体预处理组的黏着率与正常IgG对照组及阴性对照组之间均有统计学差异。pSTAT3的表达在经LIF抗体预处理的内膜中明显高于对照组。结论 LIF在小鼠胚胎着床中起着非常重要的作用,通过LIF表达的降调节可以显著降低胚胎黏着率。LIF对胚胎黏着的作用可能是通过JAK/STAT通路中STAT3的磷酸化活化而实现的。Objective To study the effect on embryo attachment rate by the down-regulated expression of LIF in a three-dimensional implantation model,and investigate the function of LIF and its pathway during embryo implantation.Methods The mice endometrium were pre-cultured in LIF antibody （0.1 and 1.0μg/ml） and normal IgG and without antibody for half an hour.Then they were co -cultured with blastocysts.The immunohistochemical staining of STAT3 and pSTAT3 were performed on antibody pre-cultured endometrium.Results There was significant different attachment rate between the high level LIF antibody group and the two control groups.The expression of pSTAT3 in endometrium pre-cultured with LIF antibody was obviously much higher than the control group.Conclusion LIF plays an important role in mouse embryo implantation.The down-regulated LIF expression could significantly decrease the attachment rate.And the role of LIF may carry out through the phosphorylation of STAT3 in the JAK/STAT pathway.

关 键 词：胚胎着床 模型 LIF STAT3 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]