不同海拔大鼠肺气肿模型肺组织caspase-3水平比较  

Comparison of Caspase 3 Lung Tissue on Rats Emphysema Model under Different Altitudes

作　　者：谌登珍 关巍[1] 冯喜英[1] 崔金霞[1] 时莹庆[1] 

机构地区：[1]青海大学附属医院,西宁810001

出　　处：《医学研究杂志》2014年第4期115-118,共4页Journal of Medical Research

摘　　要：目的观察不同海拔肺气肿大鼠肺组织caspase-3表达水平,评估低氧对肺组织凋亡的影响。方法 40只Wistar大鼠随机分为对照组(A组,6只)、中海拔盐水组(B组,6只)、中海拔肺气肿组(C组,8只)、高海拔盐水组(D组,10只)、高海拔肺气肿组(E组,10只)。A、B、C 3组大鼠饲养于西宁,海拔2260m。D组和E组饲养于低压氧舱,模拟海拔5500m。B组和D组腹腔注射生理盐水、C组和E组腹腔注射CSE,饲养4周后,采集大鼠右肺组织,免疫组化法检测肺组织caspase-3的表达。结果与A、B、D 3组比较,C组和E组肺实质破坏、部分肺泡破裂及肺泡腔扩大。5组间caspase-3细胞阳性率比较有统计学差异(P<0.001)。D组高于B组及A组(P<0.001),E组高于C组(P<0.001),C组高于B组(P<0.001),E组高于D组(P<0.001)。结论高原低氧加重了肺气肿大鼠的凋亡表达。Objective To assess the effect of hypoxia on apoptosis of lung tissue by observing emphysema rat lung tissue caspase 3 expression level at different altitudes.Methods Forty wistar rats were randomly divided into comparison group （group A,6）,Medium Altitude saline group （group B,6）,medium altitude emphysema group （group C,8）,high altitude saline group （group D,10）,high altitude pulmonary emphysema group （group E,10）.Rats of groups A,B and C were fed in Xining at 2260m altitude.Group D and group E were raised in low pressure oxygen tank,simulated 5500m altitude.Rats of group B and D were injected with saline,and group C and E were injected with CSE in their abdomens.After 4 weeks＇ feeding,immunity group collected the right lungs of the rats and did chemical test to check the expression of caspase 3 of the lung issue.Compared with groups A,B and D,lungs of rats of group C and E were almost damaged and part of the alveoli broke and alveolar space expanded.Results The results showed caspase 3 cell positive rate varies significantly among the 5 groups （P ＜ 0.001）.Cell positive rate of group D was higher than group A and B （P ＜ 0.001）,group E was higher than group C （P ＜ 0.001）,group C was higher than that of group B （P ＜ 0.001）,group E was higher than that of group D （P ＜ 0.001）.Conclusion The plateau low oxygen accelerated apoptosis expression of rats with emphysema.

关 键 词：肺气肿 低氧 凋亡 半胱氨酸天冬氨酸蛋白酶 

分 类 号：R3[医药卫生—基础医学]