阿利吉仑与氨氯地平对肾动脉狭窄大鼠血脂、肾功能及PRA影响  

Effect of Aliskiren and Amlodipine on Blood Lipid,Renal Function and PRA in Rats of Renal Artery Stenosis

作　　者：郝炎[1] 杨乔岚[1] 韩玉祯[1] 余翼[1] 陈明[1] 

机构地区：[1]四川省自贡市第一人民医院肾内科,643000

出　　处：《医学研究杂志》2014年第4期148-151,共4页Journal of Medical Research

摘　　要：目的探讨阿利吉仑与氨氯地平对肾动脉狭窄性大鼠甘油三酯、胆固醇(TC)、尿素氮、肌酐(Cr)及血浆肾素活性(plasma renin activity,PRA)的影响,拟为临床有效治疗动脉粥样硬化性肾动脉狭窄提供理论依据和策略。方法将雄性SD大鼠随机分为一肾一夹(one kidney one clip,1K1C)组、二肾一夹(two kidney one clip,2K1C)组及对照组。各组大鼠均予注射维生素D350万IU/kg并高脂饲养10周建立动脉粥样硬化(atherosclerosis,AS)合并肾动脉狭窄动物模型。再分别随机分组给予干预措施:生理盐水组:0.9%生理盐水(NS)2ml/d、阿利吉仑组:50mg(kg·d)及苯磺酸氨氯地平组:6mg/(kg·d)。4周后:心脏采血全自动生化分析仪检测甘油三酯、胆固醇、尿素氮及肌酐;放射免疫法检测血浆肾素活性。结果造模前及干预后血脂水平相比,各组大鼠胆固醇水平均显著升高(P<0.05);与生理盐水组相比较,阿利吉仑组差异有统计学意义(P<0.05),而干预后氨氯地平组差异无统计学意义(P>0.05)。造模前及干预后肌酐水平相比,生理盐水组及氨氯地平组差异均有统计学意义(P<0.05),而阿利吉仑组差异无统计学意义(P>0.05);干预后肌酐水平相比,阿利吉仑组与生理盐水组差异有统计学意义(P<0.05);而与氨氯地平组间比较差异无统计学意义(P>0.05)。比较干预后各组大鼠PRA,与生理盐水组比较,阿利吉仑组PRA均显著下降(P<0.05),而2K1C及1K1C氨氯地平组则显著升高(P<0.05)。结论阿利吉仑可显著降低PRA,亦可抑制大鼠胆固醇上升并延缓肾功能恶化,与氨氯地平相比,其对肾动脉狭窄合并动脉粥样硬化更加有益。Objective To investigate the effects of aliskiren and amlodipine on serum triglyceride,cholesterol（TC）,usea nitrogen,creatinine （Cr） and plasma renin activity （PRA） rats of glycerol renal artery stenosis,which provides a theoretics and strategies for clinical treatment of atherosclerotic renal artery stenosis.Methods Male SD rats were randomly divided into one kidney one clip （One Kidney One Clip,1K1C） group,two kidney one clip （Two Kidney One Clip,2K1C） group and control group.The rats were treated with injection of Vitamin D3 500000IU/kg and high fat diet for 10 weeks which to establish the animal model of AS with renal artery stenosis.Then the rats were divided randomly and intervened.Rats in control group were injected 0.9％ NS 2ml/d; and those in aliskiren group 50mg/（kg · d）,and in amlodipine besylate group 6mg/（kg · d）.After 4 weeks of treatment,the serum triglyceride,cholesterol,urea nitrogen and creatinine from the heart of rats were detected by automatic biochemical analyzer and the activity of plasma renin by radioimmunoassay were detected.Results Compared with the lipid levels before and after intervened,the cholesterol level in rats increased significantly （P ＜ 0.05）.Compared with the saline group,the difference of aliskiren group was significantly （P ＜ 0.05）,and the difference of amlodipine group was not significant （P ＞ 0.05）.Compared with creatinine levels before and after intervened,the differences with saline group and amlodipine group were significantly （P ＜ 0.05）,but the difference with aliskiren group was not significant（P ＞ 0.05） ;compared with creatinine levels after intervened,there was significantly difference between the aliskiren group and saline group （P ＜0.05） ; but the difference were significantly which compared with amlodipine group （P ＞ 0.05）.Compared the PRA by after intervened with the normal saline group,the PRA of aliskiren group decreased significantly （P ＜ 0.05）,while 2K1 C and 1 K1C amlodipine gr

关 键 词：肾动脉狭窄 动脉粥样硬化 阿利吉仑 苯磺酸氨氯地平 血浆肾素活性 

分 类 号：R9[医药卫生—药学]