4种提取水稻基因组DNA方法的比较  

Comparison of Four Methods of DNA Extraction from Rice

作　　者：宋敏[1] 张云孙[1] 胡卫红[1] 

机构地区：[1]云南大学生物技术系,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2001年第1期74-76,共3页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省自然科学基金资助项目(96C003Z)

摘　　要：对于含有大量硅质及多糖如酚、酯、萜等其它次生代谢产物的水稻 ,要从其组织中提取高质量的基因组DNA ,一般比较困难 .作者以水稻叶片为材料 ,分别采取了简易提取法 ,CTAB法 ,QIAGEN公司DneasyPlantMiniKits法 ,ShanghaiSangon公司GenomicDNAIsolationKits法 4种方法提取水稻基因组DNA ;并通过质量分数为 0 .8%琼脂糖凝胶电泳和PCR扩增的方法对所提取的DNA样品进行检测 ,将它们在DNA的产量、质量和耗费等方面进行简要比较 ,并根据分子生物学研究工作的实际特点 ,确定了CTAB沉淀法为最佳方法 .Rice are high in silicide,polysaccharose and secondary metabolites such as polyphenols,terpens and resins,which makes it difficult to obtain high quality genomic DNA from their tissues.Four methods,i.e basic method,CTAB precipitation,Dneasy plant Mini Kits(QIAGEN),and Genomic DNA Isolation Kits(Shanghai Sangon),were used to extract genomic DNA from the needle leaves of rice.The resulted DNA samples of four methods were tested using 0.8% agarose gel electrophoresis and PCR .The difference in yield ,quality and expense of the obtained DNA are compared,and CTAB precipitation was decided as the best method for the practical work of molecular biology.

关 键 词：基因组DNA 水稻 CTAB法 简易提取法 

分 类 号：Q943.2[生物学—植物学]